Expression of the cystathionine β synthase (CBS) gene during mouse development and immunolocalization in adult brain
Expression of the cystathionine β synthase (CBS) gene during mouse development and immunolocalization in adult brain
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DOI：
10.1177/002215540305100311
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发表时间：
2003-03-01
影响因子：
3.2
通讯作者：
London, J
中科院分区：
文献类型：
--
作者：
Robert, K;Vialard, F;London, J
由胱硫醚β合酶（CBS）缺乏引起的高同型半胱氨酸血症导致血浆同型半胱氨酸浓度升高。这是动脉粥样硬化、中风和可能的神经退行性疾病的常见风险因素。然而，由于CBS缺乏导致的高同型半胱氨酸血症与这些疾病之间的联系机制仍不清楚。早期的生物化学研究描述了各种物种中转硫作用和CBS表达的发育和成年模式。然而，关于CBS的区域和细胞表达模式，特别是在大脑中的表达模式的知识并不完整。为了完成以前的数据，我们使用原位杂交和北方印迹来表征小鼠发育过程中的CBS基因表达的空间和时间模式。在发育的早期阶段，Cbs基因仅在肝脏以及骨骼、心脏和神经系统中表达。在胚胎晚期，神经系统中的表达下降，而出生后在大脑中的表达增加，在小脑发育期间达到峰值。在成年脑中，浦肯野细胞层和海马中的表达最强。免疫组织化学分析表明，CBS蛋白定位于大脑的大部分地区，但主要是在浦肯野细胞和阿蒙的角神经元的细胞体和神经元的过程。
Hyperhomocysteinemia, caused by a lack of cystathionine beta synthase (CBS), leads to elevated plasma concentrations of homocysteine. This is a common risk factor for atherosclerosis, stroke, and possibly neurodegenerative diseases. However, the mechanisms that link hyperhomocysteinemia due to CBS deficiency to these diseases are still unknown. Early biochemical studies describe developmental and adult patterns of transsulfuration and CBS expression in a variety of species. However, there is incomplete knowledge about the regional and cellular expression pattern of CBS, notably in the brain. To complete the previous data, we used in situ hybridization and Northern blotting to characterize the spatial and temporal patterns of Cbs gene expression during mouse development. In the early stages of development, the Cbs gene was expressed only in the liver and in the skeletal, cardiac, and nervous systems. The expression declined in the nervous system in the late embryonic stages, whereas it increased in the brain after birth, peaking during cerebellar development. in the adult brain, expression was strongest in the Purkinje cell layer and in the hippocampus. Immunohistochemical analyses showed that the CBS protein was localized in most areas of the brain but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons.