Cutting edge:: Essential role of phospholipase C-γ2 in B cell development and function
Cutting edge:: Essential role of phospholipase C-γ2 in B cell development and function
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DOI：
10.4049/jimmunol.165.4.1738
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发表时间：
2000-08-15
影响因子：
4.4
通讯作者：
Kurosaki, T
中科院分区：
文献类型：
--
作者：
Hashimoto, A;Takeda, K;Kurosaki, T
B细胞Ag受体（BCR）的交联诱导多种细胞底物的酪氨酸磷酸化，包括磷脂酶C (PLC)- γ 2，它参与磷脂酰肌醇途径的激活。为了评估PLC-gamma 2在小鼠淋巴细胞生成中的重要性，我们使用ifn调控的Cre重组酶诱导了PLC-gamma 2基因的消融。新生儿诱导的PLC-gamma 2功能丧失小鼠显示成熟常规B细胞和腹膜B1细胞数量减少，体外对BCR刺激和体内对胸腺非依赖性II型Ags免疫的反应有缺陷。相比之下，T细胞发育和tcr介导的增殖正常。综上所述，PLC-gamma 2是BCR信号通路的关键组成部分，是促进B细胞发育所必需的。
Cross-linking of the B cell Ag receptor (BCR) induces the tyrosine phosphorylation of multiple cellular substrates, including phospholipase C (PLC)-gamma 2, which is involved in the activation of the phosphatidylinositol pathway. To assess the importance of PLC-gamma 2 in murine lymphopoiesis, the PLC-gamma 2 gene was inducibly ablated by using IFN-regulated Cre recombinase. Mice with a neonatally induced loss of PLC-gamma 2 function displayed reduced numbers of mature conventional B cells and peritoneal B1 cells and defective responses in vitro to BCR stimulation and in vivo to immunization with thymus-independent type II Ags. In contrast, T cell development and TCR-mediated proliferation were normal. Taken together, PLC-gamma 2 is a critical component of BCR signaling pathways and is required to promote B cell development.