Genome-wide scan for body composition in pigs reveals important role of imprinting
Genome-wide scan for body composition in pigs reveals important role of imprinting
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DOI：
10.1073/pnas.140216397
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发表时间：
2000-07-05
影响因子：
11.1
通讯作者：
Groenen, MAM
中科院分区：
文献类型：
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作者：
de Koning, DJ;Rattink, AP;Groenen, MAM
以中国梅山猪和荷兰商品猪为试验材料，研究了印记在体成分中的作用。全基因组扫描揭示了影响身体成分的五个数量性状基因座(QTL)的重要证据，其中四个是印记的。采用分离父本和母本遗传等位基因表达的统计模型进行印迹检验，在猪背脂厚的第2号染色体(SSC2)上发现了父本表达的QTL，在SSC7上发现了孟德尔表达的QTL，在SSC7的同一区域发现了影响肌肉深度的母本表达的QTL。6号染色体在短臂上有一个母系表达的QTL，在长臂上有一个父系表达的QTL，这两个QTL都影响肌内脂肪含量，单个QTL解释的表型变异在2%到10%之间。已知的与人类和老鼠的同源性没有揭示位置候选基因。这项研究表明，印记测试应该成为解开多因素性状遗传控制的标准程序。
The role of imprinting in body composition was investigated in an experimental cross between Chinese Meishan pigs and commercial Dutch pigs. A whole-genome scan revealed significant evidence for five quantitative trait loci (QTL) affecting body composition, of which four were imprinted. Imprinting was tested with a statistical model that separated the expression of paternally and maternally inherited alleles, For back fat thickness, a paternally expressed QTL was found on Sus scrofa chromosome 2 (SSC2), and a Mendelian-expressed QTL was found on SSC7, In the same region of SSC7, a maternally expressed QTL affecting muscle depth was found. Chromosome 6 harbored a maternally expressed QTL on the short arm and a paternally expressed QTL on the long arm, both affecting intramuscular fat content, The individual QTL explained from 2% up to 10% of the phenotypic variance. The known homologies to human and mouse did not reveal positional candidate genes. This study demonstrates that testing for imprinting should become a standard procedure to unravel the genetic control of multifactorial traits.