Pannexin 3 Is a Novel Target for Runx2, Expressed by Osteoblasts and Mature Growth Plate Chondrocytes
Pannexin 3 Is a Novel Target for Runx2, Expressed by Osteoblasts and Mature Growth Plate Chondrocytes
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DOI：
10.1002/jbmr.509
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发表时间：
2011-12-01
影响因子：
6.2
通讯作者：
Naus, Christian C.
中科院分区：
文献类型：
--
作者：
Bond, Stephen R.;Lau, Alice;Naus, Christian C.
泛联蛋白是一类与昆虫间隙连接蛋白同源的脊索细胞通道蛋白。泛连接蛋白家族由三个成员组成，Panx 1，Panx 2和Panx 3，这些蛋白质中的每一个在细胞过程中的作用仍在研究中。先前关于Panx 3表达的报道表明在骨骼组织中富集，因此我们通过用免疫荧光法调查发育中的小鼠胚胎进一步研究了这种分布。在颅面膜内扁骨以及附肢和中轴骨骼的长骨中检测到高水平的Panx 3。这种分布是成骨细胞和肥大软骨细胞中表达的结果。此外，Panx3启动子含有参与骨形成的转录因子的假定结合位点，我们发现碱基275和283之间的序列响应Runx2激活。总之，我们的数据表明Panx3可能在骨发育中起重要作用，并且是Runx2依赖性信号传导的新靶点。(C)2011年美国骨与矿物质研究学会。
Pannexins are a class of chordate channel proteins identified by their homology to insect gap junction proteins. The pannexin family consists of three members, Panx1, Panx2, and Panx3, and the role each of these proteins plays in cellular processes is still under investigation. Previous reports of Panx3 expression indicate enrichment in skeletal tissues, so we have further investigated this distribution by surveying the developing mouse embryo with immunofluorescence. High levels of Panx3 were detected in intramembranous craniofacial flat bones, as well as long bones of the appendicular and axial skeleton. This distribution is the result of expression in both osteoblasts and hypertrophic chondrocytes. Furthermore, the Panx3 promoter contains putative binding sites for transcription factors involved in bone formation, and we show that the sequence between bases 275 and 283 is responsive to Runx2 activation. Taken together, our data suggests that Panx3 may serve an important role in bone development, and is a novel target for Runx2-dependent signaling. (C) 2011 American Society for Bone and Mineral Research.