Role of p53 mediated miR-23a/CXCL12 pathway in osteogenic differentiation of bone mesenchymal stem cells on nanostructured titanium surfaces
Role of p53 mediated miR-23a/CXCL12 pathway in osteogenic differentiation of bone mesenchymal stem cells on nanostructured titanium surfaces
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p53介导的miR-23a/CXCL12通路在纳米结构钛表面骨髓间充质干细胞成骨分化中的作用
DOI：
10.1016/j.biopha.2019.108649
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Yuan Kai-fang
中科院分区：
文献类型：
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作者：
Zhuang Xiu-mei;Zhou Bin;Yuan Kai-fang
钛表面改性已被广泛建立，并已被证明可以改善骨整合，但。其分子机制还有待进一步阐明。microrna在调节过程中起着至关重要的作用。骨髓间充质干细胞（BMSCs）的成骨分化。在这项研究中，我们报道。miR-23a在纳米结构钛表面的骨髓间充质干细胞成骨分化过程中显著下调。miR-23a的升高抑制BMSCs的成骨分化，miR- 0.23 a的降低增强了这一过程。此外，我们还观察到CXCL12是miR-23a的直接靶点。下调CXCL12抑制纳米管Ti诱导的骨髓间充质干细胞成骨分化，效果类似。miR-23a的上调最后，在纳米管过程中p53表达降低，并调控miR-23a/CXCL12轴。钛诱导骨髓间充质干细胞成骨分化。因此，我们的研究结果表明，miR- 0.23 a通过靶向CXCL12，在纳米结构钛表面培养的骨髓间充质干细胞的成骨分化中起着至关重要的作用。这可能为骨整合提供新的临床治疗方法。
Titanium surface modification is widely established and has been proven to improve the osseointegration, but.the molecular mechanism remains to be fully elucidated. MicroRNAs serve vital roles in the process of regulating.osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). In this study, we report.that miR-23a was significantly down-regulated in the osteogenic differentiation process of BMSCs on nanostructured titanium surfaces. Elevated miR-23a inhibited osteogenic differentiation of BMSCs, and decreased miR-.23a enhanced this process. In addition, we also observed that CXCL12 was a direct target of miR-23a..Knockdown of CXCL12 inhibited nanotube Ti induced-osteogenic differentiation of BMSCs, similar to the effect.of upregulation of miR-23a. Finally, p53 was decreased and it regulated miR-23a/CXCL12 axis during nanotube.Ti induced-osteogenic differentiation of BMSCs. Therefore, our findings suggest that by targeting CXCL12, miR-.23a serves a vital role in osteogenic differentiation of BMSCs cultured on nanostructured titanium surfaces,.which may provide novel clinical treatments for osseointegration.