PROTECTION OF FERRETS AGAINST INFLUENZA CHALLENGE WITH A DNA VACCINE TO THE HEMAGGLUTININ
PROTECTION OF FERRETS AGAINST INFLUENZA CHALLENGE WITH A DNA VACCINE TO THE HEMAGGLUTININ
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DOI：
10.1016/0264-410x(94)90071-x
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发表时间：
1994-12-01
期刊：
影响因子：
5.5
通讯作者：
ROBINSON, H
中科院分区：
文献类型：
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作者：
WEBSTER, RG;FYNAN, EF;ROBINSON, H
用表达流感病毒血凝素的质粒DNA（pCMV/H1 DNA）对雪貂进行免疫，可使其在受到同源的A/PR/8/34（H1N1）流感病毒攻击时得到完全保护。通过基因枪将DNA包被的金珠递送至表皮的效率远高于肌肉注射水溶液形式的DNA。基因枪递送DNA所诱导的抗体反应比水溶液递送DNA或自然感染后产生的抗体反应具有更强的交叉反应性。这种针对流感的新型疫苗接种方法可能比自然感染提供更广泛的针对抗原漂移的保护。
Immunization of ferrets with a plasmid DNA expressing influenza virus haemagglutinin (pCMV/H1 DNA) provided complete protection from challenge with the homologous A/PR/8/34 (H1N1) influenza virus. Delivery of DNA-coated gold beads by gene gun to the epidermis was much more efficient than intramuscular delivery of DNA in aqueous solution. The antibody response induced by DNA delivered by gene gun was more cross-reactive than DNA delivered in aqueous solution or after natural infection. This novel approach to vaccination against influenza may afford broader protection against antigenic drift than that provided by natural infection.