Multi-focus parallel detection of fluorescent molecules at picomolar concentration with photonic nanojets arrays
Multi-focus parallel detection of fluorescent molecules at picomolar concentration with photonic nanojets arrays
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DOI：
10.1063/1.4896852
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发表时间：
2014-09-29
影响因子：
4
通讯作者：
Wenger, Jerome
中科院分区：
文献类型：
--
作者：
Ghenuche, Petru;de Torres, Juan;Wenger, Jerome
荧光传感和荧光相关光谱（FCS）是检测和表征单分子的强有力的方法，然而，它们的使用受到昂贵和复杂的光学仪器的限制。在这里，我们提出了一个简单的集成设计，使用自组装的二维微球阵列，实现多焦点并行检测计划的FCS。我们同时照射和收集来自几十个微球的荧光，这些微球都产生自己的光子纳米射流，以有效地激发分子并收集荧光发射。每个光子纳米喷射有助于全局检测体积，达到几十毫微微升的FCS检测体积，同时保持荧光激发和收集效率。微球光子nanojets阵列使FCS实验在低皮摩尔浓度与设备成本和对齐限制，理想的微流控芯片集成大幅降低。(C)2014 AIP Publishing LLC.
Fluorescence sensing and fluorescence correlation spectroscopy (FCS) are powerful methods to detect and characterize single molecules; yet, their use has been restricted by expensive and complex optical apparatus. Here, we present a simple integrated design using a self-assembled bi-dimensional array of microspheres to realize multi-focus parallel detection scheme for FCS. We simultaneously illuminate and collect the fluorescence from several tens of microspheres, which all generate their own photonic nanojet to efficiently excite the molecules and collect the fluorescence emission. Each photonic nanojet contributes to the global detection volume, reaching FCS detection volumes of several tens of femtoliters while preserving the fluorescence excitation and collection efficiencies. The microspheres photonic nanojets array enables FCS experiments at low picomolar concentrations with a drastic reduction in apparatus cost and alignment constraints, ideal for microfluidic chip integration. (C) 2014 AIP Publishing LLC.