HPLC analysis of γ-irradiation-induced products of tryptophan in peptides and lysozyme

HPLC analysis of γ-irradiation-induced products of tryptophan in peptides and lysozyme
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肽和溶菌酶中 γ 辐照诱导的色氨酸产物的 HPLC 分析

DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
H. Steinhart
H. Steinhart
中科院分区：
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文献类型：
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作者：
B. van Wickern;T. Simat;H. Steinhart

文献摘要

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摘要采用高效液相色谱法和紫外/荧光检测法对γ射线辐照三肽（AWA、LWL、LWM）和溶菌酶的产物进行了分析。 开发了用链霉蛋白酶E（30-60分钟，40°C）的快速和简单的一步水解，以从肽链释放辐射产物，而不损伤。 N-甲酰犬尿氨酸（NFK）、羟吲哚丙氨酸（OIA）、4-、5-、6-和7-羟色氨酸是多肽和溶菌酶辐照的主要产物。非生理性的羟色氨酸异构体4-、6-和7-羟色氨酸可能作为高蛋白含量辐照食品的标记物质。
Abstract The products of γ-irradiation of tripeptides (AWA, LWL, LWM) and lysozyme were determined by HPLC and UV/fluorescence detection. A fast and simple one-step hydrolysis with pronase E (30 – 60 min, 40°C) was developed to release the radiation products, without damage, from the peptide chain. N-Formylkynurenine (NFK), oxindolylalanine (OIA), 4-, 5-, 6- and 7-hydroxytryptophan were the main products of irradiation of peptides and lysozyme. It is possible that the nonphysiological hydroxytryptophan isomers 4-, 6- and 7-hydroxytryptophan could serve as marker substances for irradiated food with a high protein content.