ADPRibosylation of chicken red cell tubulin and inhibition of microtubule self-assembly in vitro by the NAD(+)-dependent avian ADPRibosyl transferase.
ADPRibosylation of chicken red cell tubulin and inhibition of microtubule self-assembly in vitro by the NAD(+)-dependent avian ADPRibosyl transferase.
复制标题
鸡红细胞微管蛋白的 ADP 核糖基化以及 NAD( ) 依赖性禽 ADP 核糖基转移酶对体外微管自组装的抑制。
DOI：
10.1016/0006-291x(92)91209-9
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发表时间：
1992
影响因子：
3.1
通讯作者：
Purich,DL
中科院分区：
文献类型：
--
作者：
Raffaelli,N;Scaife,RM;Purich,DL
鸡红细胞微管蛋白在ADPR转移酶的存在下，能发生NAD ~+依赖的ADP核糖基化。与霍乱和百日咳毒素催化的低水平ADPR掺入不同（即，<0.005 mol ADPR/mol微管蛋白），禽类系统显示出0.8-1.2 mol ADPR/mol微管蛋白的高得多的化学计量。修饰导致微管自组装的有效抑制，即使在牛脑微管相关蛋白的存在下或添加预组装的微管。
Chicken erythrocyte tubulin was found to undergo NAD+-dependent ADPribosylationin vitroin the presence of ADPRtransferase also isolated from avian red blood cells. Unlike the low level of ADPR incorporation catalyzed byCholeraandPertussistoxins (i.e., <0.005 mol ADPR/mol tubulin), the avian system displayed a much higher stoichiometry of 0.8–1.2 mol ADPR/mol tubulin. Modification resulted in potent inhibition of microtubule self-assembly, even in the presence of bovine brain microtubule-associated proteins or with the addition of pre-assembled microtubules.