High-Resolution Magnetic Resonance Imaging Quantitatively Detects Individual Pancreatic Islets
High-Resolution Magnetic Resonance Imaging Quantitatively Detects Individual Pancreatic Islets
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DOI：
10.2337/db11-0726
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发表时间：
2011-11-01
期刊：
影响因子：
7.7
通讯作者：
Meda, Paolo
中科院分区：
文献类型：
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作者：
Lamprianou, Smaragda;Immonen, Riikka;Meda, Paolo
我们研究了锰增强的高场磁共振成像（MEHFMRI）是否可以定量检测原位和体内的单个胰岛，并评估实验性糖尿病模型的变化。研究设计和方法-未经治疗的小鼠（n = 3），MnCl 2和葡萄糖注射小鼠（n = 6），以及链脲佐菌素注射小鼠的全胰腺（STZ; n = 4）或柠檬酸盐缓冲液（n = 4）离体成像，用于明确评价胰岛。MnCl 2和葡萄糖注射小鼠（n = 6）的外置胰腺在体内成像，以直接可视化腺体并最大限度地减少运动。在所有的情况下，MR图像采集在一个14.1 Testa扫描仪和相关的（免疫）组织学sections. MEHFMRI在离体实验中，MEHFMRI区分不同的胰腺组织和评价胰岛的相对丰度在正常血糖小鼠的胰腺。MEHFMRI还检测到STZ注射小鼠胰岛的数量和体积密度显著降低。然而，在后一种测量中，β细胞的损失在测试条件下被低估。的externalized胰腺的实验证实，MEHFMRI可以可视化本地个人的胰岛活的，麻醉mice. CONCLUSIONS-数据显示，MEHFMRI定量可视化的完整的小鼠胰腺，无论是在体内和体外的个人胰岛。糖尿病60：2853-2860，2011
OBJECTIVE-We studied whether manganese-enhanced high-field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes.RESEARCH DESIGN AND METHODS-Whole pancreata from untreated (n = 3), MnCl2 and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl2 and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Testa scanner and correlated with the corresponding (immuno)histological sections.RESULTS-In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of beta-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice.CONCLUSIONS-Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo. Diabetes 60:2853-2860, 2011