Cell-free synthesis of putative neurophysin precursors from rat and mouse hypothalamic poly (A)-RNA.
Cell-free synthesis of putative neurophysin precursors from rat and mouse hypothalamic poly (A)-RNA.
复制标题
从大鼠和小鼠下丘脑聚 (A)-RNA 中无细胞合成假定的神经素前体。
DOI：
10.1016/0006-291x(79)91869-2
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发表时间：
1979
影响因子：
3.1
通讯作者：
J. McKelvy
中科院分区：
文献类型：
--
作者：
C. Lin;P. Joseph;T. Sherman;L. Chan;J. McKelvy
从大鼠和小鼠下丘脑组织中提取了含Poly(A)的核糖核酸，并用于指导小麦胚芽提取物和兔网织红细胞裂解物在[35S]-L-半胱氨酸和[~3H]-L-脯氨酸存在下的无细胞体系中多肽的合成。翻译产物用大鼠神经内分泌蛋白的抗血清进行免疫沉淀。免疫沉淀物经蛋白A-琼脂糖层析纯化后，十二烷基硫酸钠聚丙烯酰胺凝胶电泳法显示，两种无细胞体系均可获得一种相对分子质量为17,500的单一多肽。
Poly(A)-containing RNA has been isolated from rat and mouse hypothalamic tissue and used to direct the synthesis of polypeptides in cell-free systems derived from wheat germ extract and rabbit reticulocyte lysate in the presence of [35S]-L-cysteine and [3H]-L-proline. Translation products were subjected to immunoprecipitation using an antiserum to rat neurophysin proteins. Following purification of the immunoprecipitates by protein A-Sepharose chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a single polypeptide species of molecular weight 17,500 derived from both cell-free systems.