Comparison of the probiotic effects of freshwater and marine photosynthetic bacteria in kuruma shrimp (Marsupenaeus japonicus) culture
Comparison of the probiotic effects of freshwater and marine photosynthetic bacteria in kuruma shrimp (Marsupenaeus japonicus) culture
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DOI：
10.1080/10454438.2022.2044958
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发表时间：
2022-02
影响因子：
1.2
通讯作者：
Aoi Koga;Shinsuke Takiguchi;Yusaku Tani;K. Ozaki;T. Maki;Hiroshi Okuhata;Satoshi Tanaka;S. Hayashi;Shinjiro Yamamoto;Hitoshi Miyasaka
中科院分区：
文献类型：
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作者：
Aoi Koga;Shinsuke Takiguchi;Yusaku Tani;K. Ozaki;T. Maki;Hiroshi Okuhata;Satoshi Tanaka;S. Hayashi;Shinjiro Yamamoto;Hitoshi Miyasaka
摘要 在一项田间研究中，通过使用两个商业运营的虾池，比较了一种淡水紫色非硫光合细菌（PNSB）球形红细菌（Rhodobacter sphaeroides）和一种海洋PNSB嗜硫小红卵菌（Rhodovulum sulfidophilum）对日本囊对虾（Marsupenaeus japonicus）的益生作用。对虾被投喂含有0.01%鲜重（10⁶菌落形成单位/克饲料）PNSB细胞的饲料，持续152天。海洋PNSB在收获时的平均体重、存活率（通过费舍尔精确检验，P < 0.001）和饲料转化率方面比淡水PNSB表现更好。还通过新一代测序和定量逆转录 - 聚合酶链反应比较了淡水和海洋PNSB对虾基因表达的影响。淡水和海洋PNSB都促进了几个与先天免疫系统相关的基因的表达，包括NADPH氧化酶、超氧化物歧化酶、溶菌酶、抗菌肽和凝集素，并且海洋PNSB的促进作用比淡水PNSB更强。PNSB的成本经计算为每千克饲料0.003 - 0.005美元；因此，海洋PNSB是一种用于对虾养殖的具有成本效益且可行的益生菌。
ABSTRACT In a field study, the probiotic effects of a freshwater purple nonsulfur photosynthetic bacterium (PNSB), Rhodobacter sphaeroides, and a marine PNSB, Rhodovulum sulfidophilum, on kuruma shrimp (Marsupenaeus japonicus) were compared by using two commercially operating shrimp ponds. Shrimp were fed with feed containing 0.01% fresh weight (106 cfu/g feed) of PNSB cells for 152 d. The marine PNSB showed a better performance than the freshwater PNSB in average body weight at harvest time, survival rate (P < 0.001 by Fisher’s exact test), and feed conversion rate. The effects of freshwater and marine PNSB on shrimp gene expression were also compared by next-generation sequencing and quantitative RT-PCR. Both freshwater and marine PNSB promoted the expression of several genes related to the innate immune system, including NADPH oxidase, superoxide dismutase, lysozyme, antimicrobial peptides, and lectins, and the promotive effect of marine PNSB was stronger than that of freshwater PNSB. The cost of PNSB was calculated to be US$ 0.003–0.005 per kg feed; thus, marine PNSB is a cost-effective and feasible probiotic for shrimp aquaculture.