Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor
Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor
复制标题
DOI：
10.1111/tpj.14975
复制
发表时间：
2020-09-28
期刊：
影响因子：
7.2
通讯作者：
Giuntoli, Beatrice
中科院分区：
文献类型：
--
作者：
Bui, Liem T.;Shukla, Vinay;Giuntoli, Beatrice
植物需要根据正在进行的发育计划调整它们的应激反应，以最大限度地发挥它们的功效。例如，成功的水下适应通常与节约资源和激活允许避免或衰减压力的措施的支出之间的微妙平衡有关。我们观察到拟南芥的浸没耐受性显著下降与老化有关，在发芽后发育的2到3周之间是一个关键步骤。这种对洪水的敏感性是伴随着幼年到成年的过渡而来的。转录组学分析表明，与脱落酸和氧化应激反应相关的一组基因在幼株中的表达高于成株。这些基因是由膜栓系转录因子ANAC017诱导的，而ANAC017又被淹没相关的氧化应激激活。分子、生化和遗传分析的结合表明，这些基因位于成年后向异色状态移动的基因组区域，以组蛋白H3的赖氨酸4三甲基化为标志。我们得出的结论是，虽然洪水胁迫感知和信号转导机制在幼年期和成虫期之间没有改变，但这些机制的敏感性通过表观遗传调控被整合到植物的发育程序中。
Plants need to attune their stress responses to the ongoing developmental programmes to maximize their efficacy. For instance, successful submergence adaptation is often associated with a delicate balance between saving resources and their expenditure to activate measures that allow stress avoidance or attenuation. We observed a significant decrease in submergence tolerance associated with ageing inArabidopsis thaliana, with a critical step between 2 and 3 weeks of post-germination development. This sensitization to flooding was concomitant with the transition from juvenility to adulthood. Transcriptomic analyses indicated that a group of genes related to abscisic acid and oxidative stress response was more highly expressed in juvenile plants than in adult ones. These genes are induced by the endomembrane tethered transcription factor ANAC017 that was in turn activated by submergence-associated oxidative stress. A combination of molecular, biochemical and genetic analyses showed that these genes are located in genomic regions that move towards a heterochromatic state with adulthood, as marked by lysine 4 trimethylation of histone H3. We concluded that, while the mechanisms of flooding stress perception and signal transduction were unaltered between juvenile and adult phases, the sensitivity that these mechanisms set into action is integrated, via epigenetic regulation, into the developmental programme of the plant.