Cell culture on microfabricated one-dimensional polymeric structures for bio-actuator and bio-bot applications
Cell culture on microfabricated one-dimensional polymeric structures for bio-actuator and bio-bot applications
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DOI：
10.1039/c4lc01471e
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发表时间：
2015-01-01
期刊：
影响因子：
6.1
通讯作者：
Saif, M. Taher A.
中科院分区：
文献类型：
--
作者：
Anand, Sandeep V.;Ali, M. Yakut;Saif, M. Taher A.
在这里，我们提出了一种新的1D/2D样的细胞培养聚合物平台的开发，表征和定量。该平台由锚定具有单细胞尺度（微米尺度）横截面的长（几毫米）窄丝（1D）的2D表面组成。我们将C2 C12细胞接种在平台上，并与2D培养相比，表征其迁移、增殖和分化模式。我们发现，细胞降落在2D表面上，然后迁移到细丝只有当2D表面已成为接近融合。随机接近细丝的单个和孤立的细胞总是朝着2D表面缩回。一旦在细丝上，与在2D基底上相比，它们向肌管的分化加快。肌管产生周期性的抽搐力，使细丝变形，在尖端产生超过17 μ m的位移。这种鞭毛运动可以用来开发自主的微型生物机器人。
Here, we present the development, characterization and quantification of a novel 1D/2D like polymeric platform for cell culture. The platform consists of a 2D surface anchoring a long (few millimeters) narrow filament (1D) with a single cell scale (micro scale) cross section. We plate C2C12 cells on the platform and characterize their migration, proliferation, and differentiation patterns in contrast to 2D culture. We find that the cells land on the 2D surface, and then migrate to the filament only when the 2D surface has become nearly confluent. Individual and isolated cells randomly approaching the filament always retract away towards the 2D surface. Once on the filament, their differentiation to myotubes is expedited compared to that on 2D substrate. The myotubes generate periodic twitching forces that deform the filament producing more than 17 mu m displacement at the tip. Such flagellar motion can be used to develop autonomous micro scale bio-bots.