Model for Probing Membrane-Cortex Adhesion by Micropipette Aspiration and Fluctuation Spectroscopy
Model for Probing Membrane-Cortex Adhesion by Micropipette Aspiration and Fluctuation Spectroscopy
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DOI：
10.1016/j.bpj.2015.02.027
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发表时间：
2015-04-21
影响因子：
3.4
通讯作者：
Sens, Pierre
中科院分区：
文献类型：
--
作者：
Alert, Ricard;Casademunt, Jaume;Sens, Pierre
我们提出了一个模型，膜皮质粘附耦合膜变形，流体动力学和动力学的膜皮质配体。在其最简单的形式，该模型给出了明确的预测膜分离的临界压力和粘附能的值。我们表明，这些数量表现出显着的依赖于活性acto-myosin应力。该模型提供了一个简单的框架，通过微管实验获得定量信息的皮层活动。我们还扩展了模型，将波动和显示，膜-皮层耦合的稳定性的详细信息，可以通过微管抽吸和波动光谱测量的组合。
We propose a model for membrane-cortex adhesion that couples membrane deformations, hydrodynamics, and kinetics of membrane-cortex ligands. In its simplest form, the model gives explicit predictions for the critical pressure for membrane detachment and for the value of adhesion energy. We show that these quantities exhibit a significant dependence on the active acto-myosin stresses. The model provides a simple framework to access quantitative information on cortical activity by means of micropipette experiments. We also extend the model to incorporate fluctuations and show that detailed information on the stability of membrane-cortex coupling can be obtained by a combination of micropipette aspiration and fluctuation spectroscopy measurements.