Different expressions of aquaporin water channels and macrophages infiltration in human cervix remodeling during pregnancy
Different expressions of aquaporin water channels and macrophages infiltration in human cervix remodeling during pregnancy
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DOI：
10.1093/biolre/ioab191
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发表时间：
2021-10
影响因子：
3.6
通讯作者：
Jinying Yang;Shengjun Yu;Guanglan Zhang;Zheng Zheng-Zheng;Ping Li;S. Mei;Xinjia Han
中科院分区：
文献类型：
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作者：
Jinying Yang;Shengjun Yu;Guanglan Zhang;Zheng Zheng-Zheng;Ping Li;S. Mei;Xinjia Han
尽管水通道蛋白水通道（aquaporin water channels, AQPs）在维持女性生殖道水分平衡中发挥着关键作用，并随着妊娠的进展促使宫颈水肿中水分含量逐渐增加，但其与巨噬细胞浸润和胶原蛋白含量在人宫颈重塑中的关系尚需进一步研究。本研究首次在人宫颈成熟过程中同时检测AQP3、AQP4、AQP5、AQP8和巨噬细胞的表达和定位。孕26周（GD26W）时，这些AQPs的免疫反应性比GD6W和GD15W高2.6 ~ 6倍，但GD39W上AQP4的表达在GD15W上下降幅度相似，其他AQPs在GD39W上继续上升。在GD26W上，AQP3、AQP4和AQP5的表达强度在颈间质中比在血管周围更丰富；AQP3、AQP5、AQP8在颈间质中的分布与GD39W上血管周围区域的分布相同。与GD15W相比，GD26W组上皮下区巨噬细胞数量高1.7倍，中心区巨噬细胞数量高3.0倍；这些数字在GD39W上仍然很高。电镜图显示，GD26W和GD39W组的宫颈伸伸性明显增加，巨噬细胞浸润增加，宫颈含水量增加，胶原纤维间隙增大。提示妊娠期人子宫颈AQPs表达上调与巨噬细胞浸润增强密切相关；它们之间可能存在一种正反馈机制，导致含水量的增加和胶原蛋白的降解。人宫颈重构中AQPs表达的增加与炎症反应增强、巨噬细胞浸润密切相关；他们之间可能存在积极的反馈机制。
Abstract Despite aquaporin water channels (AQPs) play a critical role in maintaining water homeostasis in female reproductive tract and prompt a gradual increase in water content in cervical edema as pregnancy progressed, their relationship with macrophage infiltration and collagen content in human cervical remodeling need to be further investigated. This is the first study to examine the expression and localization of AQP3, AQP4, AQP5, AQP8, and macrophages simultaneously in human cervical ripening. The immunoreactivity of these AQPs was 2.6 to 6-fold higher on gestational weeks 26 (GD26W) than that on GD6W and GD15W, but AQP4 expression on GD39W dropped a similar extent on GD15W, other AQPs continued to rise on GD39W. The AQP3, AQP4, and AQP5 intensity seemed more abundant in cervical stroma than in the perivascular area on GD26W; the distribution of AQP3, AQP5, and AQP8 in cervical stroma was equivalent to that in the perivascular area on GD39W. Macrophage numbers were 1.7-fold higher in subepithelium region and 3.0-fold higher in center area on GD26W than that on GD15W; such numbers remained elevated on GD39W. The electron micrographs showed that cervical extensibility increased significantly on GD26W and GD39W accompanied with increased macrophage infiltration, cervical water content, and much more space among collagen fibers. These findings suggest that the upregulation of AQPs expression in human cervix is closely related to enhanced macrophage infiltration during pregnancy; there may be a positive feedback mechanism between them to lead the increase of water content and the degradation of collagen. Summary sentence The increase of AQPs expression in human cervix remodeling is closely related to enhanced inflammatory responses and macrophage infiltration; there may be a positive feedback mechanism between them.