Influence of KGF on the progression of pancreatic cancer.
Influence of KGF on the progression of pancreatic cancer.
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DOI：
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发表时间：
2009-08
影响因子：
2
通讯作者：
X. Zang;M. Lerner;D. Brackett;J. Pento
中科院分区：
文献类型：
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作者：
X. Zang;M. Lerner;D. Brackett;J. Pento
背景角质形成细胞生长因子(KGF)促进多种上皮细胞的增殖和运动，在胰腺癌中表达上调。本研究验证了KGF可能通过促进胰腺癌细胞的增殖和运动而启动或促进胰腺癌进展的假说。材料与方法KGF作用24小时和48小时后，采用体外细胞损伤实验检测HPAF-II胰腺癌细胞的迁移和增殖情况。用免疫组织化学方法确定KGF受体(KGFR)在这些细胞中的定位。结果KGF可显著增强HPAF-II细胞的增殖和运动能力。此外，KGF还增强了这些癌细胞的运动形态。结论KGF能迅速影响HPAF-II细胞的增殖和运动能力，提示KGF可能参与了胰腺癌的发生发展。
BACKGROUND Keratinocyte growth factor (KGF increases the proliferation and motility of many epithelial cells and is known to be up-regulated in pancreatic cancer. The present study examined the hypothesis that KGF may initiate or enhance the progression of pancreatic cancer by increasing the proliferation and motility of pancreatic cancer cells. MATERIALS AND METHODS HPAF-II pancreatic cancer cell migration and proliferation was evaluated using a culture wounding assay 24 and 48 hours following KGF treatment. KGF receptor (KGFR) localization in these cells was established by immunohistochemistry. RESULTS KGF treatment significantly increased the proliferation and motility of the HPAF-II cells. In addition, KGF enhanced the motile morphology of these cancer cells. CONCLUSION The results of this study indicate that KGF has a rapid influence on the proliferation and motility of HPAF-II cells and suggest that KGF may be involved in the progression of pancreatic cancer.