Accelerating DNA nanomotor by branched DNAzyme for ultrasensitive optical detection of thrombin
Accelerating DNA nanomotor by branched DNAzyme for ultrasensitive optical detection of thrombin
复制标题
通过分支 DNAzyme 加速 DNA 纳米马达，用于凝血酶的超灵敏光学检测
DOI：
10.1016/j.microc.2018.03.004
复制
发表时间：
2018-06-01
影响因子：
4.8
通讯作者：
Chen, Junbo
中科院分区：
文献类型：
--
作者：
Du, Huan;Yang, Peng;Chen, Junbo
虽然基于DNA组装的纳米机器或纳米器件已经得到了深入的研究，但DNA组装的缓慢动力学会影响DNA纳米机器的应用。在此，我们将一个分支的DNAzyme整合到合成的DNA马达中，从而显著加速了DNA马达的运行。与单个DNAzyme构建的DNA马达相比，分支DNAzyme DNA纳米马达具有更快的操作特性。该方法可在45 min内检测到低至100 fM的凝血酶，为DNA纳米管在临床诊断中的分析应用提供了极大的潜力。(C)2018爱思唯尔B.V.保留所有权利。
Although DNA assembly-based nanomachines or nanodevice have been intensively studied, the application of DNA nanomachines can be suffered from the slow kinetic of DNA assembly. Herein, we integrated a branched DNAzyme into synthetic DNA motors, thus significantly accelerating the operation of DNA motors. Compared with DNA motors built by a single DNAzyme, the branched DNAzyme DNA nanomotor has much faster operation features. For the application, it could detect as low as 100 fM thrombin within 45 min. The strategy endowed the DNA nanomotor a great potentiality to the analytical application in clinical diagnosis. (C) 2018 Elsevier B.V. All rights reserved.