QTL mapping for leaf area in maize (Zea mays L.) under multi-environments
QTL mapping for leaf area in maize (Zea mays L.) under multi-environments
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DOI：
10.1016/s2095-3119(16)61524-1
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发表时间：
2017-01-01
影响因子：
4.8
通讯作者：
Liu Jian-chao
中科院分区：
文献类型：
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作者：
Cui Ting-ting;He Kun-hui;Liu Jian-chao
叶片是绿色植物光合作用的主要器官。叶面积对玉米干物质积累和籽粒产量起着至关重要的作用。因此，研究叶面积的遗传基础将有助于玉米高产育种。本研究以玉米自交系徐178与K12杂交获得的150个F-7重组自交系为材料，在多种环境条件下测定了3个穗叶面积(TELA)。利用包含复合区间作图法(ICIM)定位了Tela在单一环境下的数量性状基因座(QTL)和估计育种值(EBV)。在单一环境条件下共检测到8个QTL，其中4个对EBV也可在单一环境中检测到。这表明EBV检测到的QTL具有较高的遗传稳定性。位于2.04/2.05号染色体上的主效QTL(qTELA_2-9)在4个环境中均可检测到，且具有较高的表型贡献率(10.79%~16.51%)，是分子育种的理想目标。此外，还利用联合分析揭示了叶面积在6个环境中的遗传基础。共鉴定出6个QTLx环境互作和9个上位性互作。结果表明，叶面积的遗传基础不仅受加性效应的影响，还受上位性效应、环境互作效应的影响。
Leaves are the main organs of photosynthesis in green plants. Leaf area plays a vital role in dry matter accumulation and grain yield in maize (Zea mays L.). Thus, investigating the genetic basis of leaf area will aid efforts to breed maize with high yield. In this study, -a total of 150 F-7 recombinant inbred lines (RILs) derived from a cross between the maize lines Xu 178 and K12 were used to evaluate three ear-leaves area (TELA) under multi-environments. Inclusive composite interval mapping (ICIM) was used to identify quantitative trait loci (QTLs) for TELA under a single environment and estimated breeding value (EBV). A total of eight QTLs were detected under a single environmental condition, and four QTLs were identified for EBV which also can be detected in single environment. This indicated that the EBV-detected QTLs have high genetic stability. A major QTL (qTELA_2-9) located in chromosome bin 2.04/2.05 could be detected in fourenvironments and has a high phenotypic contribution rate (ranging from 10.79 to 16.51%) that making it a good target for molecular breeding. In addition, joint analysis was used to reveal the genetic basis of leaf area in six environments. In total, six QTLxenvironment interactions and nine epistatic interactions were identified. Our results reveal that the genetic basis of the leaf area is not only mainly determined by additive effects, but also affected by epistatic effects environmental interaction effects.