Frequency of double minute chromosomes and combined cytogenetic abnormalities and their characteristics
Frequency of double minute chromosomes and combined cytogenetic abnormalities and their characteristics
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双微小染色体的频率和组合细胞遗传学异常及其特征
DOI：
10.1007/s13353-010-0007-z
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发表时间：
2011-02-01
影响因子：
2.4
通讯作者：
Fu, Songbin
中科院分区：
文献类型：
--
作者：
Fan, Yihui;Mao, Renfang;Fu, Songbin
双微体染色体（DM）是染色体外基因组扩增的细胞遗传学标志。原发性癌症中DM的频率和DM阳性原发性癌症病例的细胞遗传学特征在很大程度上是未知的。为了解决这些问题，我们检索了Mitelman数据库，并分析了所有DM阳性的原发癌核型（787个核型）。DM的总频率为1.4%（787例DM阳性病例;总计54，398例）。我们发现，肾上腺癌（28.6%，地形）和神经母细胞瘤（31.7%，形态）中DM的频率最高。DM在每个肿瘤中的频率远低于以前的报告。DM在恶性肿瘤中的频率显著高于良性肿瘤，这证实了DM是恶性细胞遗传学标记。DM合并细胞遗传学异常通过主成分分析（PCA）被识别并分为两组，一组包含−4、−5、−8、−9、−10、−13、−14、−15、−16、−17、−18、−20、−21和−22，另一组包含− 1 p、− 5 q、+7和+20。DM阳性癌症病例中突出的不平衡是染色体丢失。然而，来自不同形态癌症的DM阳性癌症病例不能明确地分为亚组。我们的大型数据库分析提供了新的知识DM及其合并细胞遗传学异常的原发性癌症。
Double minute chromosomes (DMs) are the cytogenetic hallmark of extra-chromosomal genomic amplification. The frequency of DMs in primary cancer and the cytogenetic features of DMs-positive primary cancer cases are largely unknown. To unravel these issues, we retrieved the Mitelman database and analyzed all DMs-positive primary cancerous karyotypes (787 karyotypes). The overall frequency of DMs is 1.4% (787 DMs-positive cases; total 54,398 cases). We found that DMs have the highest frequency in adrenal carcinoma (28.6%, topography) and neuroblastoma (31.7%, morphology). The frequencies of DMs in each tumor were much lower than in previous reports. The frequency of DMs in malignant cancers is significantly higher than in benign cancers, which confirms that DMs are malignant cytogenetic markers. DMs combined cytogenetic abnormalities are identified and sorted into two groups by principal component analysis (PCA), with one group containing −4, −5, −8, −9, −10, −13, −14, −15, −16, −17, −18, −20, −21, and −22, and the other containing −1p, −5q, +7, and +20. The prominent imbalance in DMs-positive cancer cases is chromosome loss. However, DMs-positive cancer cases, deriving from different morphologic cancers, cannot be clearly divided into subgroups. Our large database analysis provides novel knowledge of DMs and their combined cytogenetic abnormalities in primary cancer.