REGULATION OF CARBOHYDRATE COMPOSITION OF SACCHAROMYCES-CEREVISIAE UNDER GROWTH LIMITATION
REGULATION OF CARBOHYDRATE COMPOSITION OF SACCHAROMYCES-CEREVISIAE UNDER GROWTH LIMITATION
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DOI：
10.1007/bf00425203
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发表时间：
1972-01-01
期刊：
影响因子：
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通讯作者：
FIECHTER, A
中科院分区：
文献类型：
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作者：
KUENZI, MT;FIECHTER, A
1.研究了在恒化器中好氧培养的酵母菌在不同生长速率下的碳水化合物组成。并与储备糖途径酶的比活性进行了比较。在葡萄糖限制下，储备碳水化合物的合成随着底物供应限制的增加而强烈增加，即，增长率下降。发酵细胞只积累糖原，而生长较慢的生物体则具有纯氧化代谢，含有海藻糖和大量糖原。2.在出芽过程中，纯呼吸细胞降解部分储备。在过量底物存在下，储备碳水化合物含量低，在细胞周期中保持不变。3.在出芽周期中，细胞中结构碳水化合物的百分比变化不大，4.在0.077 h-1的生长速率下，1葡萄糖限制下培养的细胞比氮限制下培养的细胞含有更高量的储备。葡萄糖焦磷酸化酶和糖原磷酸化酶作为生长速率的函数遵循储备碳水化合物的生长速率。
1.The carbohydrate composition ofSaccharomyces cerevisiaecultivated aerobically in the chemostat at different growth rates was investigated. It was compared with the specific activities of enzymes of the reserve carbohydrate pathways. Under glucose limitation the synthesis of reserve carbohydrates increases strongly with increasing limitation of substrate supply, i.e., decreasing growth rate. Fermenting cells accumulate only glycogen, whereas more slowly growing organism with a purely oxidative metabolism contain trehalose and high amounts of glycogen.2.During the budding process the purely respirative cells degrade part of their reserves. In the presence of excess substrate the reserve carbohydrate content is low and remains unchanged during the cell cycle.3.The percentage of structural carbohydrates in the cells shows little changes during the budding cycle, being scarcely influence by the growth rate.4.At a growth rate of 0.077 h-1the cells cultivated under glucose limitation contain even a higher amount of reserves than those grown under nitrogen limitation.5.The course of the specific activities of UDP-glucose pyrophosphorylase and glycogen phosphorylase as a function of the growth rate follows that of the reserve carbohydrates.