Caveolin-1 localization in Muller cells of the retina
Caveolin-1 localization in Muller cells of the retina
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DOI：
10.1267/ahc.35.423
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发表时间：
2002-01-01
影响因子：
2.4
通讯作者：
Ueda, H
中科院分区：
文献类型：
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作者：
Ueda, H
大约十年前，人们发现了一种新的小窝蛋白，小窝蛋白，并且从那时起，小窝功能的许多新方面被发现。然而，它仍然是难以捉摸的小窝蛋白在眼组织中的功能，所以我们想解决这个问题。我们进行了实验，在体内和体外的免疫组织化学/细胞化学和免疫印迹。免疫组化结果显示，Caveolin-1在大鼠视网膜的Muller细胞和角膜内皮细胞中均有表达。共聚焦双标记图像显示caveolin-1与L-谷氨酸/L-天冬氨酸转运蛋白（GLAST）和谷氨酰胺合成酶（GS）共定位，证实caveolin-1在Muller细胞中表达。免疫印迹显示培养的Muller细胞表达小窝蛋白-1和-2，但不表达小窝蛋白-3，免疫细胞化学显示小窝蛋白-1和-2在Muller细胞中的定位不同。此外，小窝蛋白-1是专门与GLAST，但不与GS在培养的穆勒细胞共定位。这些结果表明，小窝蛋白-1可能与谷氨酸代谢在视网膜中通过穆勒细胞的可能性。
Novel caveolae proteins, caveolins, were found about a decade ago, and many new aspects of caveolae functions have since been uncovered. However, it remains elusive how caveolins function in ocular tissues, so we would like to address this issue. We conducted experiments in vivo and in vitro with immunohisto/cytochemistry and immunoblot. In vivo immunohistochemistry showed that caveolin-1 was expressed in Muller cells and corneal endothelial cells of the rat retina. Confocal double-labeling images demonstrated that caveolin-1 was colocalized with L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS), confirming that caveolin-1 is expressed in Muller cells. With in vitro experiments, immunoblotting showed that cultured Muller cells express caveolin-1 and -2 but not -3, and immunocytochemistry demonstrated that caveolin-1 and -2 are differently localized in Muller cells. Moreover, caveolin-1 is exclusively co-localized with GLAST but not with GS in cultured Muller cells. These results indicate the possibility that caveolin-1 may be associated with glutamate metabolism in the retina through Muller cells.