CLONING, STRUCTURE, CELLULAR-LOCALIZATION, AND POSSIBLE FUNCTION OF THE TUMOR-SUPPRESSOR GENE LETHAL(3)MALIGNANT BLOOD NEOPLASM-1 OF DROSOPHILA-MELANOGASTER
CLONING, STRUCTURE, CELLULAR-LOCALIZATION, AND POSSIBLE FUNCTION OF THE TUMOR-SUPPRESSOR GENE LETHAL(3)MALIGNANT BLOOD NEOPLASM-1 OF DROSOPHILA-MELANOGASTER
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DOI：
10.1006/dbio.1994.1126
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发表时间：
1994-05-01
影响因子：
2.7
通讯作者：
GATEFF, E
中科院分区：
文献类型：
--
作者：
KONRAD, L;BECKER, G;GATEFF, E
黑腹果蝇的肿瘤抑制基因，致死（3）恶性血液肿瘤-1（+），是吞噬血细胞类型（浆细胞）分化所必需的。在同源突变状态下，它导致这些血细胞的恶性转化。我们在这里提出的克隆，测序，结构和表达的l（3）mbn-1（+）基因在发育过程中。通过生殖系转化、产生回复突变体以及检测血细胞和其他组织中相应的mRNA来鉴定克隆的基因。推测的MBN（83）蛋白C端富含G-S，与人细胞角蛋白K1、K10和小鼠兜甲蛋白具有同源性，并对野生型l（3）mbn-1（+）基因的结构和可能的功能进行了讨论。(C)1994年出版社出版。
The tumor suppressor gene, lethal (3) malignant blood neoplasm-1(+), of Drosophila melanogaster is required for the differentiation of the phagocytic blood-cell type, the plasmatocyte. In the homozygously mutated state it causes the malignant transformation of these blood cells. We present here the cloning, sequencing, structure, and expression of the l(3)mbn-1(+) gene during development. The cloned gene was identified by germ-line transformation, generation of revertants, and the detection of the corresponding mRNA in blood cells and other tissues. Homologies of the G-S-rich C-terminus of the putative MBN(83) protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1(+) gene are discussed. (C) 1994 Academic Press, Inc.