Static tension is associated with increased smooth muscle cell DNA synthesis in rat pulmonary arteries.
Static tension is associated with increased smooth muscle cell DNA synthesis in rat pulmonary arteries.
复制标题
静态张力与大鼠肺动脉中平滑肌细胞 DNA 合成的增加有关。
DOI：
10.1152/ajpheart.1995.268.3.h1133
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Orton,EC
中科院分区：
文献类型：
--
作者：
Weiser,MC;Majack,RA;Tucker,A;Orton,EC
在肺动脉高压的发展过程中，血管细胞增殖与肺血管内压的升高密切相关。在肺动脉高压中，压力升高可能对诱导血管细胞增殖产生的直接物理效应尚不清楚。为了解决这个问题，静态力（0，1，5，和10克）施加到在无血清培养基中培养的肺门肺动脉环。胸苷类似物，溴脱氧尿苷（BrdU），到中膜和外膜细胞的掺入进行了分析，免疫组化。中膜细胞BrdU掺入（阳性细胞%）增加（P < 0.0001）与0 g对照组相比，在所有力水平下（未安装和已安装，但未施加力）（未安装：0.65 +/- 0.08;安装：0 g，1.8 +/- 0.39; 1 g，3.7 +/- 0.35; 5 g，5.2 +/- 0.43; 10 g，2.8 +/- 0.17）。与对照动脉（6.0 +/- 0.54）相比，动脉的缺氧暴露和内皮剥脱减弱（P < 0.05）张力诱导的中膜细胞BrdU标记（分别为2.5 +/- 0.96和3.3 +/- 0.63）。硝苯地平降低张力诱导的中膜细胞BrdU掺入（P < 0.05）。不同压力下外膜细胞DNA合成无明显差异，但缺氧使外膜细胞BrdU掺入量显著降低（P < 0.05）。我们的结论是，静态壁张力可能是一个重要的直接刺激中膜细胞DNA合成。
During the development of pulmonary hypertension, vascular cell proliferation closely parallels the rise in pulmonary intravascular pressure. The possible direct physical effect that elevated pressures may have on inducing vascular cell proliferation in pulmonary hypertension is unclear. To address this question, static force (0, 1, 5, and 10 g) was applied to hilar pulmonary arterial rings cultured in a serum-free medium. Incorporation of the thymidine analogue, bromodeoxyuridine (BrdU), into medial and adventitial cells was analyzed by immunohistochemistry. Medial cell BrdU incorporation (%positive cells) was increased (P < 0.0001) at all levels of force compared with 0-g controls (unmounted and mounted, but without applied force) (unmounted: 0.65 +/- 0.08; mounted: 0 g, 1.8 +/- 0.39; 1 g, 3.7 +/- 0.35; 5 g, 5.2 +/- 0.43; 10 g, 2.8 +/- 0.17). Hypoxia exposure and endothelial denudation of arteries attenuated (P < 0.05) tension-induced medial cell BrdU labeling (2.5 +/- 0.96 and 3.3 +/- 0.63, respectively) compared with control arteries (6.0 +/- 0.54). Nifedipine reduced tension-induced medial cell BrdU incorporation (P < 0.05). There was no difference in DNA synthesis in adventitial cells at the various levels of force, although hypoxia decreased adventitial cell BrdU incorporation overall (P < 0.05). We conclude that static wall tension may be an important direct stimulus for medial cell DNA synthesis.