Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.
Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.
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ColE1 质粒的 DNA 链起始序列在单链 DNA 噬菌体中的表达。
DOI：
10.1073/pnas.77.11.6566
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发表时间：
1980
影响因子：
11.1
通讯作者：
Ray,DS
中科院分区：
文献类型：
--
作者：
Nomura,N;Ray,DS
为了研究ColE1质粒h链（后链）复制的起始机制，将ColE1起始区片段（Hae II-E）插入到丝状DNA噬菌体M13的基因间区进行克隆。在克隆片段的l链（前导链）上检测到一个能够促进单链DNA模板上DNA链起始的位点。这个位点被命名为rri-1利福平抗性起始)，在利福平存在的情况下，指导嵌合噬菌体单链DNA向亲本复制形式的转化，这阻断了M13互补链起源的功能。rri-1的功能依赖于dnaG和dnaB基因产物。假设在ColE1 DNA的单向复制过程中，rri-1可能是滞后DNA链合成的起始位点。
In order to investigate initiation of H-strand (lagging strand) replication of the plasmid ColE1, the origin region fragment (Hae II-E) of ColE1 was inserted into the intergenic region of filamentous DNA phage M13 and cloned. A site capable of promoting DNA strand initiation on a single-stranded DNA template has been detected on the L-strand (leading strand) of the cloned fragment. The site, named rri-1 rifampicin-resistant initiation), directs conversion of chimeric phage single-stranded DNA to parental replicative form in the presence of rifampicin, which blocks the function of the complementary strand origin of M13. The function of rri-1 is dependent on both the dnaG and dnaB gene products. It is postulated that rri-1 might be an initiation site for synthesis of the lagging DNA strand during unidirectional replication of ColE1 DNA.