Transmissible gastroenteritis virus induces apoptosis in swine testicular cell lines but not in intestinal enterocytes
Transmissible gastroenteritis virus induces apoptosis in swine testicular cell lines but not in intestinal enterocytes
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DOI：
10.1053/jcpa.2000.0386
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发表时间：
2000-07-01
影响因子：
0.8
通讯作者：
Chae, C
中科院分区：
文献类型：
--
作者：
Kim, B;Kim, O;Chae, C
在体外通过感染猪睾丸细胞和体内通过感染仔猪肠道组织寻找普渡传染性胃肠炎病毒株（TGEV）感染引起细胞凋亡的证据。使用的方法是(1)DNA电泳检测DNA片段，(2)末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸荧光素刻划和标记（TUNEL）。仅在tgev感染的ST患者中检测到DNA“阶梯”。TUNEL法在tgv感染的ST细胞中检测到许多凋亡迹象，阳性（深棕色）染色反应存在于大多数细胞核中，没有背景染色。在接种仔猪后不同时间，tgev感染的肠细胞未见这种染色。因此，感染TGEV的仔猪肠细胞似乎不会发生细胞凋亡。(C) 2000 Harcourt出版社有限公司
Evidence of apoptosis caused by infection with the Purdue strain of transmissible gastroenteritis virus (TGEV) was sought in vitro tin infected swine testicular [ST] cells) and in vivo tin the intestinal tissues of infected piglets). The methods used were (1) DNA electrophoresis for detection of DNA fragmentation, and (2) terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick and labelling (TUNEL). DNA "laddering" was detected in TGEV-infected ST tells only. Numerous signs of apoptosis were detected in TGEV-infected ST cells by TUNEL assay, the positive (dark brown) staining reaction being: present in the majority of cell nuclei, without background staining. No such staining tvas seen in TGEV-infected enterocytes at various times after inoculation of piglets. Thus, it would appear that apoptosis does not occur in the enterocytes of piglets infected with TGEV. (C) 2000 Harcourt Publishers Ltd.