In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography
In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography
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DOI：
10.1364/opex.13.006286
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发表时间：
2005-08-08
期刊：
影响因子：
3.8
通讯作者：
Boccara, C
中科院分区：
文献类型：
--
作者：
Grieve, K;Dubois, A;Boccara, C
我们提出了一种新的高速全视场光学相干断层扫描（OCT）仪器，第一个全视场OCT系统，能够在体内眼部成像。由于使用氙弧灯源和林尼克型干涉仪中的相对高数值孔径显微镜物镜，实现了类似于1 μ m的各向同性分辨率。全视场照明允许并行捕获二维正面图像，使用快速CMOS相机作为检测器阵列。每个正面图像在4ms周期内以250 Hz的最大重复率采集。每个正面图像的检测灵敏度为71 dB。通过图像相关和平均可以实现更高的灵敏度，尽管帧速率降低。我们目前的第一个初步结果，在大鼠眼前段的体内成像，揭示了一些细胞功能的角膜层。(C)2005年，美国光学学会。
We present a new high speed full-field optical coherence tomography (OCT) instrument, the first full-field OCT system that is capable of in vivo ocular imaging. An isotropic resolution of similar to 1 mu m is achieved thanks to the use of a xenon arc lamp source and relatively high numerical aperture microscope objectives in a Linnik-type interferometer. Full-field illumination allows the capture of two-dimensional en face images in parallel, using a fast CMOS camera as detector array. Each en face image is acquired in a 4 ms period, at a maximum repetition rate of 250 Hz. Detection sensitivity per en face image is 71 dB. Higher sensitivity can be achieved by image correlation and averaging, although frame rate is reduced. We present the first preliminary results of in vivo imaging in the anterior segment of the rat eye, which reveal some cellular features in the corneal layers. (C) 2005 Optical Society of America.