High efficiency enzymatic synthesis of triglycerides with varying chain length and degree of unsaturation in a solvent-free system under vacuum
High efficiency enzymatic synthesis of triglycerides with varying chain length and degree of unsaturation in a solvent-free system under vacuum
复制标题
在真空下无溶剂系统中高效酶法合成不同链长和不饱和度的甘油三酯
DOI：
10.1002/jctb.6389
复制
发表时间：
2020-07-01
影响因子：
3.4
通讯作者：
Zhang, Lu
中科院分区：
文献类型：
--
作者：
Bi, Yanlan;Chen, Jiali;Zhang, Lu
具有不同链长和不饱和度的甘油三酯现在在食品和制药工业中有很高的需求。在甘油三酯的商业生产中，酶催化合成方法优于基于化学的方法。该研究发现，真空的应用可以提高使用Novozym 435（EC 3.1.1.3）（来自南极假丝酵母的固定化脂肪酶B）酶促合成各种甘油三酯的效率和生产率。结果真空条件不适用于短链脂肪酸（C4 ~ C6）的酯化反应，反应过程中脂肪酸损失较多。在常压下，C-4-C-6饱和脂肪酸的转化率较低（15-35%）。在真空条件下，甘油与几种中链和长链（C-8-C-18）饱和脂肪酸的酯化反应实现了高转化率（>95%）和甘油三酯产率（约90%）。脂肪酸的不饱和度也影响甘油三酯的得率。由C18：2n-6合成的甘油三酯含量（76.17 ± 0.45%）显著低于C18：0（91.59 ± 1.58%）和C18：1 n-9（90.77 ± 0.85%）（P < 0.05）。此外，高氧化度的脂肪酸对甘油三酯合成有负面影响。该真空无溶剂系统允许合成酶重复使用11次，而不显着降低脂肪酶活性的酯化脂肪acids.Conclusion研究的真空促进甘油三酯合成过程提供了一个经济上有吸引力的，但可持续的解决方案，用于生产广泛的甘油三酯产品。(c)2020化学工业协会
BACKGROUND Triglycerides with varying chain length and unsaturation degree are now in high demand in the food and pharmaceutical industries. Enzymatic catalyzed synthesis methods are favored over chemical-based processes in the commercial production of triglycerides. This study found that the application of vacuum can improve the efficiency and productivity of enzymatic synthesis of various triglycerides using Novozym 435 (EC 3.1.1.3), an immobilized lipase B from Candida antarctica. The system was used for esterification of fatty acids varying in chain length, unsaturation degree, and oxidation degree.RESULTS The vacuum condition was not applicable to short chain fatty acids (C-4-C-6) due to loss of fatty acids in the reaction. The conversions of C-4-C-6 saturated fatty acids were low (15-35%) under atmospheric pressure. Under vacuum, high conversions (>95%) and triglyceride yields (approximately 90%) were achieved in the esterification of glycerol with several medium-chain and long-chain (C-8-C-18) saturated fatty acids. Moreover, unsaturation degree of fatty acids also affected the triglyceride yield. The triglyceride contents synthesized from C18:2n-6 (76.17 +/- 0.45%) was significantly lower (P < 0.05) than those from C18:0 (91.59 +/- 1.58%) and C18:1n-9 (90.77 +/- 0.85%). Furthermore, fatty acids with a high oxidation degree had negative effect on triglyceride synthesis. This vacuum solvent free system allowed the synthesis enzyme to be reused 11 times without significant decrease in the lipase activity for esterification of fatty acids.CONCLUSION The studied vacuum facilitated triglyceride synthesis process provides an economically attractive yet sustainable solution for the production of a wide range of triglyceride products. (c) 2020 Society of Chemical Industry