The Sertoli cell expressed gene secernin-1 (Scrn1) is dispensable for male fertility in the mouse.
The Sertoli cell expressed gene secernin-1 (Scrn1) is dispensable for male fertility in the mouse.
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DOI：
10.1002/dvdy.299
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发表时间：
2021-07
影响因子：
2.5
通讯作者：
O'Bryan, Moira K.
中科院分区：
文献类型：
--
作者：
Houston, Brendan J.;Nagirnaja, Liina;Merriner, D. Jo;O'Connor, Anne E.;Okuda, Hidenobu;Omurtag, Kenan;Smith, Craig;Aston, Kenneth I.;Conrad, Donald F.;O'Bryan, Moira K.
男性不育症是一种普遍的临床表现，由于精子发生所需的数千个基因，其可能具有很强的遗传成分。在这项研究中，我们研究了基因 Scrn1 在男性生育力中的作用。根据单细胞 RNA 测序，Scrn1 在性别决定期间优先在 XY 性腺中表达，并在成年支持细胞中优先表达。我们研究了 Scrn1 在幼年和成年组织中的表达，并生成了敲除小鼠模型来测试其在雄性生育力中的作用。 Scrn1 在出生后睾丸检查的所有年龄段都有表达，但其表达在出生后 7-14 天达到峰值，并且 SCRN1 蛋白明显定位于支持细胞。 Scrn1 缺失是通过去除外显子 3 来实现的，其缺失对男性生育力或性别决定没有影响。基因敲除小鼠能够产生与野生型小鼠相同大小的后代，并产生相同数量的具有相当活力和形态特征的精子。人们发现 Scrn1 对于男性生育能力是可有可无的，但这项研究将 SCRN1 确定为支持细胞细胞质的新标记。
Male infertility is a prevalent clinical presentation for which there is likely a strong genetic component due to the thousands of genes required for spermatogenesis. Within this study we investigated the role of the gene Scrn1 in male fertility. Scrn1 is preferentially expressed in XY gonads during the period of sex determination and in adult Sertoli cells based on single cell RNA sequencing. We investigated the expression of Scrn1 in juvenile and adult tissues and generated a knockout mouse model to test its role in male fertility. Scrn1 was expressed at all ages examined in the post-natal testis, however its expression peaked at postnatal day 7–14 and SCRN1 protein was clearly localized to Sertoli cells. Scrn1 deletion was achieved via removal of exon 3, and its loss had no effect on male fertility or sex determination. Knockout mice were capable of siring litters of equal size to wild type counterparts and generated equal numbers of sperm with comparable motility and morphology characteristics. Scrn1 was found to be dispensable for male fertility, but this study identifies SCRN1 as a novel marker of the Sertoli cell cytoplasm.
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睾丸生殖细胞肿瘤中癌睾基因的综合表征
影响因子：
4
作者：
Chang, Yuting;Wang, Xuewei;Hu, Zhibin
通讯作者：
Hu, Zhibin
影响因子：
3.6
作者：
Bouma, Gerrit J.;Hudson, Quanah J.;Eicher, Eva M.
通讯作者：
Eicher, Eva M.
影响因子：
5.3
作者：
通讯作者：
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影响因子：
1.6
作者：
Gaikwad, Avinash S.;Loh, Khai Lee;O'Bryan, Moira K.
通讯作者：
O'Bryan, Moira K.
DOI：
10.1073/pnas.1015935108
发表时间：
2011-04-26
影响因子：
11.1
作者：
Gibbs, Gerard M.;Orta, Gerardo;O'Bryan, Moira K.
通讯作者：
O'Bryan, Moira K.