Syndeins: the spectrin-binding protein(s) of the human erythrocyte membrane.
Syndeins: the spectrin-binding protein(s) of the human erythrocyte membrane.
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突蛋白：人红细胞膜的血影蛋白结合蛋白。
DOI：
10.1073/pnas.76.5.2340
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发表时间：
1979
影响因子：
11.1
通讯作者：
S. Goodman
中科院分区：
文献类型：
--
作者：
J. Yu;S. Goodman
人红细胞膜十二烷基硫酸钠/聚丙烯酰胺凝胶中所有主要条带的二维胰蛋白酶和胰凝乳蛋白酶分析表明，每条带都有一个特征图。而2.1带（T. L. Steck）和该带以下的几种多肽显示出相似的胰蛋白酶和胰凝乳蛋白酶肽图谱，因此似乎是一个密切相关的蛋白质或降解产物家族。此外，它们都含有由来自两个已知血影蛋白结合片段的肽所占的肽的子集。我们发现，这两个片段来自2.1相关的蛋白质，并得出结论，带2.1及其相关的蛋白质，我们命名为“syndeins”，结合血影蛋白，并将其连接到红细胞膜。
Two-dimensional tryptic and chymotryptic analyses of all the major bands in a sodium dodecyl sulfate/polyacrylamide gel of the human erythrocyte membrane show that each band has a characteristic map. However, band 2.1 (nomenclature of T. L. Steck) and several polypeptides below this band exhibit similar tryptic and chymotryptic peptide maps and thus appear to be a family of closely related proteins or degradation products. Furthermore, they all contain a subset of peptides that are accounted for by the peptides from two known spectrin-binding fragments. We show that both fragments derive from 2.1-related proteins and conclude that band 2.1 and its related proteins, which we name "syndeins", bind spectrin and connect it to the erythrocyte membrane.