Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1
Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1
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DOI：
10.1007/s10532-004-5023-4
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发表时间：
2005-10
期刊：
影响因子：
3.6
通讯作者：
M. Sasaki;J. Maki;K. Oshiman;Y. Matsumura;T. Tsuchido
中科院分区：
文献类型：
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作者：
M. Sasaki;J. Maki;K. Oshiman;Y. Matsumura;T. Tsuchido
研究了鞘氨醇单胞菌对双酚A [BPA; 2，2-双（4-羟基苯基）丙烷]的降解能力和降解途径。菌株AO 1，分离自日本的蔬菜种植领域的土壤中，进行了研究。该菌株能够在含有BPA作为唯一碳源的基础矿物盐培养基（BSMB培养基）中生长，并且能够在L培养基中在6小时内降解115μg ml− 1 BPA。利用高效液相色谱（HPLC）在培养上清中检测到BPA代谢产物，并通过GC-MS和LC-MS-MS进行了鉴定。BPA降解的基础矿物盐培养基中的细胞诱导BPA，活性检测仅在细胞内可溶性组分中的辅酶，如NADH，NAD+，NADPH或NADP+的存在下。在BSMB培养基中加入细胞色素P450抑制剂甲吡酮，BPA降解和细胞生长减少。细胞内可溶性组分的BPA降解活性也受到细胞色素P450抑制剂的抑制。一氧化碳差谱表明细胞色素P450存在于细胞中，细胞色素P450的量对应于细胞的BPA降解活性。我们的结果为细胞色素P450系统参与鞘氨醇单胞菌的BPA代谢提供了证据。菌株AO 1。
The capacity and pathway of bisphenol A [BPA; 2,2-bis(4-hydroxyphenyl)propane] degradation inSphingomonassp. strain AO1, which was isolated from the soil of a vegetable-growing field in Japan, were investigated. The bacterial strain was able to grow in a basal mineral salt medium containing BPA as the sole carbon source (BSMB medium), and was able to degrade 115μg ml−1BPA in 6 h in L medium. Several BPA metabolites were detected in the culture supernatant by HPLC and then identified by GC-MS and LC-MS-MS. These compounds were confirmed to be the same as those reported for other BPA-degrading bacteria. BPA degradation by cells in the basal mineral salt medium was induced by BPA, and activity was detected only in the intracellular soluble fraction in the presence of coenzymes, such as NADH, NAD+, NADPH or NADP+. The addition of metyrapone, a cytochrome P450 inhibitor, to BSMB medium resulted in a decrease in BPA degradation and cell growth. The BPA-degradation activity of the intracellular soluble fraction was also inhibited by the cytochrome P450 inhibitor. Carbon monoxide difference spectra indicated that cytochrome P450 was present in the cells and that the amount of cytochrome P450 corresponded to the cellular BPA-degradation activity. Our results provide evidence that the cytochrome P450 system is involved in BPA metabolism inSphingomonassp. strain AO1.