The metabolism of arsenite and arsenate by the rat.
The metabolism of arsenite and arsenate by the rat.
复制标题
大鼠对亚砷酸盐和砷酸盐的代谢。
DOI：
10.1016/s0272-0590(83)80145-6
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Clarkson,TW
中科院分区：
文献类型：
--
作者：
Lerman,S;Clarkson,TW
亚砷酸盐和亚砷酸盐在大鼠体内的代谢王晓明，陈晓华，等.北京：北京大学出版社，1998.应用程序。毒素。3：309-314。研究了亚砷酸盐和亚砷酸盐在大鼠体内及大鼠肝、肾切片和肝细胞中的分布和代谢差异。静脉注射后五分钟。给雄性SD大鼠灌胃4.8nmol亚砷酸盐或亚砷酸盐，血砷水平仅为初始剂量的10%。血液中的砷水平随后上升：在4小时内，大约67%的初始剂量的亚砷酸盐和28%的初始剂量的砷酸盐在血舱内。红细胞中砷的主要形态为二甲基砷酸(DMA)。亚砷酸盐迅速分布到肝脏和肾脏，砷只迅速分布到肾脏。在接触亚砷酸盐4小时后，肝脏切片摄取的砷是砷暴露后的6倍，肾脏切片摄取的砷是砷暴露后的2倍。分离的肝细胞在亚砷酸盐暴露后摄取高达20倍的砷。在染砷组大鼠肝切片和肝细胞培养上清液中检测到DMA，而在染砷组肝切片和肝细胞培养上清液中几乎未检测到DMA。然而，在接触砷的肾脏切片的培养液中发现的DMA比在肝脏切片的培养液中多5倍。磷酸盐抑制肾切片对砷的吸收和代谢。这些研究表明，无机砷迅速被肝脏和肾脏摄取并甲基化。亚砷酸盐被两个器官甲基化，而亚砷酸盐可能只被肾脏甲基化。
The Metabolism of Arsenite and Arsenate by the Rat. Lerman, S.A. and Clarkson, T.W. (1983).Fundam. App. Toxicol.3: 309–314. Differences in distribution and metabolism of arsenite and arsenate were studied in rats and in rat liver and kidney slices and hepatocytes. Five minutes after i.v. administration of 4.8 nmol arsenite or arsenate to male Sprague-Dawley rats, blood levels of arsenic were only 10 percent of the initial dose. Blood arsenic levels then rose: by 4 hours about 67 percent of the initial dose of arsenite and 28 percent of the initial dose of arsenate were in the blood compartment. The predominant form of arsenic in the RBC was dimethylarsinic acid (DMA). Arsenite was rapidly distributed to both liver and kidney; arsenate was rapidly distributed to kidney only. After 4 hours of exposure to arsenite, liver slices had taken up six times more arsenic and kidney slices two times more arsenic than after exposure to arsenate. Isolated hepatocytes took up as much as 20 times more arsenic after arsenite exposure. DMA was found in the medium of the liver slices and hepatocytes exposed to arsenite, but very little DMA was found in the medium of the arsenate-exposed liver slices and hepatocytes. However, five times more DMA was found in the medium of the kidney slices exposed to arsenate than in the medium of the liver slices. Phosphate inhibited uptake and metabolism of arsenate by kidney slices. These studies indicate that inorganic arsenic is rapidly taken up by liver and kidney and methylated. Arsenite is methylated by both organs, whereas arsenate may be methylated by kidney only.