Transcriptional deregulation of stress-growth balance in Nicotiana benthamiana biofactories producing insect sex pheromones.
Transcriptional deregulation of stress-growth balance in Nicotiana benthamiana biofactories producing insect sex pheromones.
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DOI：
10.3389/fpls.2022.941338
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
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植物生物工厂是可持续生产高价值化合物的一个有前途的平台，其中包括昆虫性信息素，这是农业中常规杀虫剂的绿色替代品。最近，我们已经构建了转基因本氏烟草（Nicotiana benthamiana）植物（“Sexy Plants”，SxP），其成功地产生蛾（鳞翅目）性信息素化合物（Z）-11-十六碳烯-1-醇和（Z）-11-十六碳烯乙酸酯的共混物。然而，性信息素的有效生物合成导致生长和发育惩罚，降低了SxP在生物制造中的商业用途的潜力。为了深入了解潜在的分子反应，我们分析了全基因组转录组，并评估了它与V1.0和V1.2 SxP系的低产量和高产量转基因植物的生长和信息素生产的关系。在我们的研究中，高产的SxPv1.2植物积累了最高量的信息素，但与v1.0高产植物相比，仍然保持了更好的生长。为了对转录组数据进行深入的生物学解释，我们准备了一个全面的功能性N。benthamiana基因组注释以及基因翻译到拟南芥，使功能信息转移通过使用拟南芥知识网络。差异基因表达分析，对比信息素生产者野生型植物，揭示了虽然只有少数基因差异调节低产量的植物，高产量的植物表现出巨大的转录重编程。他们表现出类似压力的反应，表现为光合作用相关基因的下调和激素信号和次级代谢相关基因的表达显着差异，后者可能导致以前报道的挥发性变化。进一步的网络分析证实了与茉莉酸的激活和赤霉酸信号的下调的应激样反应，阐明了观察到的生长惩罚不仅仅是一个更高的代谢负担的结果施加在异源生物合成途径的组成型表达的可能性，而是信号通路扰动的结果。我们的工作提出了一个全面的转录组学分析的不利压力信号在N。本萨米亚纳生物工厂，可应用于其他生物生产系统。
Plant biofactories are a promising platform for sustainable production of high-value compounds, among which are insect sex pheromones, a green alternative to conventional insecticides in agriculture. Recently, we have constructed transgenic Nicotiana benthamiana plants (“Sexy Plants”, SxP) that successfully produce a blend of moth (Lepidoptera) sex pheromone compounds (Z)-11-hexadecen-1-ol and (Z)-11-hexadecenyl acetate. However, efficient biosynthesis of sex pheromones resulted in growth and developmental penalty, diminishing the potential for commercial use of SxP in biomanufacturing. To gain insight into the underlying molecular responses, we analysed the whole-genome transcriptome and evaluated it in relation to growth and pheromone production in low- and high-producing transgenic plants of v1.0 and v1.2 SxP lines. In our study, high-producing SxPv1.2 plants accumulated the highest amounts of pheromones but still maintained better growth compared to v1.0 high producers. For an in-depth biological interpretation of the transcriptomic data, we have prepared a comprehensive functional N. benthamiana genome annotation as well as gene translations to Arabidopsis thaliana, enabling functional information transfer by using Arabidopsis knowledge networks. Differential gene expression analysis, contrasting pheromone producers to wild-type plants, revealed that while only a few genes were differentially regulated in low-producing plants, high-producing plants exhibited vast transcriptional reprogramming. They showed signs of stress-like response, manifested as downregulation of photosynthesis-related genes and significant differences in expression of hormonal signalling and secondary metabolism-related genes, the latter presumably leading to previously reported volatilome changes. Further network analyses confirmed stress-like response with activation of jasmonic acid and downregulation of gibberellic acid signalling, illuminating the possibility that the observed growth penalty was not solely a consequence of a higher metabolic burden imposed upon constitutive expression of a heterologous biosynthetic pathway, but rather the result of signalling pathway perturbation. Our work presents an example of comprehensive transcriptomic analyses of disadvantageous stress signalling in N. benthamiana biofactory that could be applied to other bioproduction systems.
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影响因子：
7.4
作者：
Agrawal S;Karcher D;Ruf S;Erban A;Hertle AP;Kopka J;Bock R
通讯作者：
Bock R
影响因子：
7.4
作者：
Attaran, Elham;Major, Ian T.;Howe, Gregg A.
通讯作者：
Howe, Gregg A.
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.
影响因子：
5.6
作者：
Diego-Martin B;González B;Vazquez-Vilar M;Selma S;Mateos-Fernández R;Gianoglio S;Fernández-Del-Carmen A;Orzáez D
通讯作者：
Orzáez D
影响因子：
3.5
作者：
Garcia-Perez E;Diego-Martin B;Quijano-Rubio A;Moreno-Giménez E;Selma S;Orzaez D;Vazquez-Vilar M
通讯作者：
Vazquez-Vilar M