Changes in bioactive lipids, alkylacylglycerol and ceramide, occur in HIV-infected cells.
Changes in bioactive lipids, alkylacylglycerol and ceramide, occur in HIV-infected cells.
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生物活性脂质、烷基酰基甘油和神经酰胺的变化发生在感染 HIV 的细胞中。
DOI：
10.1016/s0006-291x(05)81480-9
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发表时间：
1992
影响因子：
3.1
通讯作者：
Bell,RM
中科院分区：
文献类型：
--
作者：
VanVeldhoven,PP;Matthews,TJ;Bolognesi,DP;Bell,RM
在HIV感染的CEM细胞中测量已知调节信号转导或具有其他生物活性的生物活性脂质的质量水平。甘油二酯，蛋白激酶C的激活剂，以及烷基酰基甘油的水平升高。一个更剧烈的增加，观察到在HIV感染后的神经酰胺水平，而鞘氨醇水平几乎没有影响。有趣的是，变化的幅度与感染时间有关，在感染后8天高于4天。这些脂质在HIV感染的细胞病变效应中的可能作用进行了讨论。此外，一种改进的方法来定量同时在粗脂质提取物中的二酰基甘油和烷基酰基甘油，基于他们的磷酸化由E。colidiacylglycerol kinase，colidiacylglycerol。
The mass levels of bioactive lipids known to modulate signal transduction or to possess other biological activities were measured in HIV-infected CEM cells. The levels of diacylglycerol, an activator of protein kinase C, as well as of alkylacylglycerol were elevated. A more drastic increase was observed in the ceramide levels after HIV-infection, whereas sphingosine levels were hardly influenced. Interestingly, the magnitude of the changes was related to the infection time, being higher at 8 days after infection then at 4 days. The possible role of these lipids in the cytopathic effects of HIV-infection is discussed.In addition, an improved methodology to quantitate simultaneously diacylglycerol and alkylacylglycerol in crude lipid extracts, based upon their phosphorylation byE. colidiacylglycerol kinase, is presented.