Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant
Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant
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DOI：
10.1111/j.1574-6941.2006.00268.x
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发表时间：
2007-04-01
影响因子：
4.2
通讯作者：
Manaia, Celia M.
中科院分区：
文献类型：
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作者：
da Silva, Miguel Ferreira;Vaz-Moreira, Ivone;Manaia, Celia M.
在 18 个月的时间里，从市政处理厂的原始废水（189 个分离株）和处理后的废水（156 个分离株）中分离出了肠杆菌。这些分离株被鉴定为埃希氏菌属（76%）、志贺氏菌属（7%）、克雷伯氏菌属（12%）和不动杆菌属（4%）。采用琼脂扩散法测定抗生素阿莫西林、庆大霉素、环丙沙星、磺胺甲恶唑/甲氧苄啶、四环素和头孢噻吩，消毒剂过氧化氢、次氯酸钠、季铵/甲醛和碘，以及重金属镍、镉、铬、汞和锌。使用引物CS5和CS3通过PCR扩增检测1类整合子。与原水相比，处理后的废水中大肠杆菌的相对比例较高。分离株对环丙沙星和头孢噻吩耐药（分别为 P < 0.0001 和 P < 0.05）。除汞与四环素和磺胺甲恶唑/甲氧苄啶呈正相关外，抗生素、消毒剂和重金属耐药性之间未观察到显着的正相关。 1 类整合子的可变区，在 c 中检测到。 10% 的埃希氏菌属。分离株，主要含有基因盒 aadA1/dhfrI。
Over 18 months, enterobacteria were isolated from the raw (189 isolates) and treated (156 isolates) wastewater of a municipal treatment plant. The isolates were identified as members of the genera Escherichia (76%), Shigella (7%), Klebsiella (12%) and Acinetobacter (4%). Antimicrobial susceptibility phenotypes were determined using the agar diffusion method for the antibiotics amoxicillin, gentamicin, ciprofloxacin, sulfamethoxazole/trimethoprim, tetracycline and cephalothin, the disinfectants hydrogen peroxide, sodium hypochlorite, quaternary ammonium/formaldehyde and iodine, and the heavy metals nickel, cadmium, chromium, mercury and zinc. Class 1 integrons were detected by PCR amplification using the primers CS5 and CS3. Compared with the raw influent, the treated wastewater presented higher relative proportions of Escherichia spp. isolates resistant to ciprofloxacin and cephalothin (P < 0.0001 and P < 0.05, respectively). Except for mercury, which showed a positive correlation with tetracycline and sulfamethoxazole/trimethoprim, no significant positive correlations were observed between antibiotic, disinfectant and heavy metal resistance. The variable regions of class 1 integrons, detected in c. 10% of the Escherichia spp. isolates, contained predominantly the gene cassettes aadA1/dhfrI.