A simple and compact smartphone accessory for quantitative chemiluminescence-based lateral flow immunoassay for salivary cortisol detection
A simple and compact smartphone accessory for quantitative chemiluminescence-based lateral flow immunoassay for salivary cortisol detection
复制标题
DOI：
10.1016/j.bios.2014.08.048
复制
发表时间：
2015-02-15
影响因子：
12.6
通讯作者：
Roda, Aldo
中科院分区：
文献类型：
--
作者：
Zangheri, Martina;Cevenini, Luca;Roda, Aldo
我们开发了一种简单而准确的生物传感器，基于化学发光 (CL) 侧流免疫分析 (LFIA) 方法，集成在智能手机中，用于定量检测唾液皮质醇。该生物传感器基于使用过氧化物酶-皮质醇缀合物的直接竞争性免疫测定，通过添加化学发光底物鲁米诺/增强剂/过氧化氢进行检测。智能手机摄像头用作光探测器，通过特定应用程序进行图像采集和数据处理。我们 3D 打印简单的配件来适应智能手机。该系统包括一个装有 LFIA 条的墨盒，以及一个带有钢琴凸透镜和墨盒插入槽的智能手机适配器。这在相机和 LFIA 膜之间提供了一个微型暗箱和对齐的光学接口，用于采集 CL 信号。该方法简单、快速，检测限为0.3 ng/mL。它提供0.3-60 ng/mL范围内的定量分析，足以检测临床可接受范围内的唾液皮质醇。因此，它可以在用于临床生物标志物监测的家庭自诊断设备技术不断增长的领域找到应用，克服当前利用智能手机连接和所含相机增强性能的传统系统获取敏感和定量信息的困难。 (C) 2014 Elsevier B.V. 保留所有权利。
We have developed a simple and accurate biosensor based on a chemiluminescent (CL)-lateral flow immunoassay (LFIA) method integrated in a smartphone to quantitatively detect salivary cortisol. The biosensor is based on a direct competitive immunoassay using peroxidase-cortisol conjugate, detected by adding the chemiluminescent substrate luminol/enhancer/hydrogen peroxide. The smartphone camera is used as light detector, for image acquisition and data handling via a specific application. We 3D-printed simple accessories to adapt the smartphone. The system comprises a cartridge, which houses the LFIA strip, and a smartphone adaptor with a piano-convex lens and a cartridge-insertion slot. This provides a mini-darkbox and aligned optical interface between the camera and the LFIA membrane for acquiring CL signals. The method is simple and fast, with a detection limit of 0.3 ng/mL. It provides quantitative analysis in the range of 0.3-60 ng/mL, which is adequate for detecting salivary cortisol in the clinically accepted range. It could thus find application in the growing area of home-self-diagnostic device technology for clinical biomarker monitoring, overcoming the current difficulties in achieving sensitive and quantitative information with conventional systems taking the advantage of smartphone connectivity and the enhanced performance of the included camera. (C) 2014 Elsevier B.V. All rights reserved.