The Tyrosinase-Encoding Gene of Lentinula edodes, Letyr, Is Abundantly Expressed in the Gills of the Fruit-Body during Post-Harvest Preservation
The Tyrosinase-Encoding Gene of Lentinula edodes, Letyr, Is Abundantly Expressed in the Gills of the Fruit-Body during Post-Harvest Preservation
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DOI：
10.1271/bbb.80810
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发表时间：
2009-05-01
影响因子：
1.6
通讯作者：
Uchimiya, Hirofumi
中科院分区：
文献类型：
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作者：
Sato, Toshitsugu;Kanda, Katsuhiro;Uchimiya, Hirofumi
香菇子实体在保存过程中的鳃褐变被认为是由酪氨酸酶催化的黑色素生物合成所致。我们从香菇白腐担子菌Lentinula edodes（香菇）中分离出一个基因组DNA序列和编码一种假定的酪氨酸酶的cDNA。该基因名为Letyr，由一个1854 bp的开放阅读框组成，由8个内含子打断，编码一个由618个氨基酸残基组成的蛋白质，估计分子质量为68 kDa。已知参与铜结合结构域的氨基酸残基在推导出的LeTyr氨基酸残基中是保守的。在收获后保存期间，子实体鳃中Letyr的转录和翻译表达增加。Letyr表达与果体保存之间的相关性表明酪氨酸酶基因表达有助于鳃褐变。
The gill browning of Lentinula edodes fruit-bodies during preservation is thought to be due to melanin biosynthesis catalyzed by tyrosinase. We isolated a genomic DNA sequence and cDNA encoding a putative tyrosinase from the white rot basidiomycete Lentinula edodes (shiitake mushroom). The gene, named Letyr, consists of a 1,854-bp open reading frame interrupted by eight introns, and encodes a putative protein of 618 amino acid residues with an estimated molecular mass of 68 kDa. Amino acid residues known to be involved in copper-binding domains were conserved in the deduced amino acid residues of LeTyr. Transcriptional and translational expression of Letyr in the gills of the fruit-body increased during preservation after harvest. This correlation between Letyr expression and fruit-body preservation suggests that tyrosinase gene expression contributes to gill browning.