Preclinical evaluation of heat-denatured [18F]FDG-labeled red blood cells for detecting splenic tissues with PET in rats
Preclinical evaluation of heat-denatured [18F]FDG-labeled red blood cells for detecting splenic tissues with PET in rats
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DOI：
10.1016/j.nucmedbio.2017.09.002
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Jinzaki, Masahiro
中科院分区：
文献类型：
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作者：
Matsusaka, Yohji;Nakahara, Tadaki;Jinzaki, Masahiro
引言：热变性Tc-99 m标记的红细胞（RBC）用于通过放射性造影术检测脾组织。本研究旨在评估使用热变性[F-18]氟脱氧葡萄糖的可行性（[F-18] FDG）标记的红细胞在使用正电子发射断层扫描（PET）检测大鼠脾组织中的作用。用磷酸盐缓冲盐水洗涤RBC，在38 ℃下用[F-18]FDG标记，并在50 ℃下热变性15分钟。通过在37 ℃下孵育0-180分钟期间测量细胞外放射性来评估体外稳定性。进行细胞外液的薄层色谱（TLC）。4只大鼠静脉注射自体红细胞，同时进行PET扫描30 min，在PET图像上分析包括脾脏在内的多个器官的时间-活性曲线。标记RBC释放低水平放射性180 min。TLC显示80%的释放放射性是由于[F-18]DG-6-磷酸。全身图像显示，所有4只大鼠注射后不久，脾脏中热变性[F-18] FDG标记的RBC 5均出现强烈摄取。时间活性曲线显示，脾脏摄取持续增加30分钟，在其他器官的放射性的量，除了膀胱，减少后的初始surgeon.Conclusions：热变性[F-18] FDG标记的红细胞是合适的脾脏特异性剂PET。该方法作为热变性Tc-99 m标记的红细胞荧光造影的替代方法具有临床相关性。(C)2017爱思唯尔公司All rights reserved.
Introduction: Heat-denatured Tc-99m-labeled red blood cells (RBCs) are used for detecting splenic tissues with scintigraphy. The present study aimed to evaluate the feasibility of using heat-denatured [F-18]fluorodeoxyglucose ([F-18] FDG)-labeled RBCs in detecting splenic tissues using positron emission tomography (PET) in rats.Methods: RBCs were washed with phosphate buffered saline, labeled with [F-18]FDG at 38 degrees C, and heat-denatured at 50 degrees C for 15 min. In vitro stability was assessed by measuring extracellular radioactivity during the 0-180 min incubation at 37 degrees C. Thin layer chromatography (TLC) of the extracellular fluid was performed. The autologous RBCs were intravenously injected in four rats and PET scanning was simultaneously performed for 30 min. Time-activity curves of several organs, including the spleen, were analyzed on the PET images.Results: Labeling efficiency was 92%. Low levels of radioactivity were released from the labeled RBCs for 180 min. TLC revealed that 80% of the released radioactivity was due to [F-18]DG-6-phosphate. Whole body images showed strong uptake of heat-denatured [F-18]FDG-labeled RBC5 in the spleen soon after injection in all four rats. Time activity curves revealed that the splenic uptake continued to increase for 30 min and the amount of radioactivity in the other organs, except the urinary bladder, decreased after the initial surge.Conclusions: Heat-denatured [F-18]FDG-labeled RBCs are suitable spleen-specific agents for PET. This method is clinically relevant as an alternative for heat-denatured Tc-99m-labeled RBC scintigraphy. (C) 2017 Elsevier Inc. All rights reserved.