Evolutionary Relationships of Flying Foxes (Genus Pteropus) in the Philippines Inferred from DNA Sequences of Cytochrome b Gene
Evolutionary Relationships of Flying Foxes (Genus Pteropus) in the Philippines Inferred from DNA Sequences of Cytochrome b Gene
复制标题
从细胞色素b基因DNA序列推测菲律宾狐蝠（Pteropus）的进化关系
DOI：
10.1023/a:1015161305843
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发表时间：
2002
影响因子：
2.4
通讯作者：
Takao Namikawa
中科院分区：
文献类型：
--
作者：
S. T. Bastian;S. T. Bastian;K. Tanaka;R. Anunciado;N. G. Natural;A. C. Sumalde;Takao Namikawa
使用完整的细胞色素b基因序列（1140 bp）对六种狐蝠属（四种来自菲律宾）进行了研究，以推断它们的进化关系。使用邻接法、简约法和最大似然法分析通过聚合酶链式反应生成的 DNA 序列。我们估计这些翼龙物种中的第一次进化事件大约发生在 13.90 ± 1.49 MYA 之前。在这段短暂的进化时间内，我们进一步假设，在菲律宾发现的狐蝠的祖先经历了随后的多样化，在拓扑结构中形成了两个簇。第一个簇由P组成。 pumilus（菲律宾特有），P. spiosus（仅限于棉兰老岛西部）与 P.肩胛骨，而第二个包括P。吸血鬼和P。 dasymallusspecies 基于第一和第二密码子位置的分析。一致地，所有系统发育分析都揭示了 P. 的密切关联。 dasymalluswithP. vampyrus与之前的报告分类相矛盾。 Dasymallus under subniger 种组与 P.短须，P.斯佩修斯，P.黑色素减少。菲律宾特有种（P. pumilus）与P. pumilus 密切相关。壮观。 P 的代表性样本。 vampyrus表现出1.87%的较大遗传距离。 P之间的遗传距离较大。达西马鲁斯和 P. hypermelanus、P. pumilus 和 P. spiosus 表示一个独特的物种组。
Six flying fox species, genusPteropus(four from the Philippines) were investigated using completecytochrome bgene sequences (1140 bp) to infer their evolutionary relationships. The DNA sequences generated via polymerase chain reaction were analyzed using the neighbor-joining, parsimony, and maximum likelihood methods. We estimated that the first evolutionary event among thesePteropusspecies occurred approximately 13.90 ± 1.49 MYA. Within this short period of evolutionary time we further hypothesized that the ancestors of the flying foxes found in the Philippines experienced a subsequent diversification forming two clusters in the topology. The first cluster is composed ofP. pumilus(Philippine endemic),P. speciosus(restricted in western Mindanao) withP. scapulatus, while the second one comprisedP. vampyrusandP. dasymallusspecies based on the analysis from first and second codon positions. Consistently, all phylogenetic analyses divulged close association ofP. dasymalluswithP. vampyruscontradicting the previous report categorizingP. dasymallusundersubnigerspecies group withP. pumilus,P. speciosus, andP. hypomelanus. The Philippine endemic species (P. pumilus) is closely linked withP. speciosus. The representative samples ofP. vampyrusshowed a large genetic distance of 1.87%. The large genetic distance betweenP. dasymallusandP. hypomelanus, P. pumilusandP. speciosusdenotes a distinct species group.