CHARACTERIZATION AND MOLECULAR-CLONING OF A HUMAN PARVOVIRUS GENOME
CHARACTERIZATION AND MOLECULAR-CLONING OF A HUMAN PARVOVIRUS GENOME
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DOI：
10.1126/science.6095448
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
TATTERSALL, P
中科院分区：
文献类型：
--
作者：
COTMORE, SF;TATTERSALL, P
与红细胞再生障碍性贫血和红斑感染性红斑(第五种疾病)血清学相关的小型人类病毒的基因组被证明是具有自启动发夹末端的线性、非置换的单链DNA分子，这是细小病毒科基因组的特征。将人细小病毒染色体分子克隆到细菌载体上，用DNA：DNA杂交的方法研究其与其他哺乳动物细小病毒血清型的亲缘关系。它与人类腺相关病毒没有关系，但确实显示出与啮齿动物辅助非依赖性细小病毒基因组的遥远进化关系。这强烈地表明它是一种自主的细小病毒，因此是这组常见动物病原体中导致人类疾病的第一个例子。
The genome of the small human virus serologically associated with erythrocyte aplasia and erythema infectiosum (fifth disease) is shown to be a linear, nonpermuted, single-stranded DNA molecule with self-priming hairpin termini, properties which are characteristic of the genomes of the family Parvoviridae. This human parvovirus chromosome was molecularly cloned into bacterial plasmid vectors and the cloned DNA was used to explore its relatedness to other mammalian parvovirus serotypes by DNA:DNA hybridization. It is not related to the human adeno-associated viruses but does show a distant evolutionary relationship to genomes of the helper-independent parvoviruses of rodents. This strongly suggests that it is an autonomous parvovirus, and as such is the first example of a member of this group of common animal pathogens to cause disease in man.