Corelease of GABA/glycine in lamina-X of the spinal cord of neonatal rats
Corelease of GABA/glycine in lamina-X of the spinal cord of neonatal rats
复制标题
新生大鼠脊髓X层GABA/甘氨酸的共释放
作者：
Riad Seddik;R. Schlichter;J. Trouslard
在全细胞记录条件下，用膜片钳技术记录新生大鼠脊髓切片上的LINA-X神经元。层-X环绕脊髓中央管，并含有中央自主神经核的交感节前神经元。在河豚毒素和犬尿酸存在下，记录到微小的抑制性突触后电流，分别阻断动作电位依赖的递质释放和谷氨酸能传递。我们记录了-氨基-正丁酸/甘氨酸的混合微小突触电流，这表明-氨基-正丁酸和甘氨酸可以从相同的单个突触小泡中共同释放，并且这种共同释放可以被突触后细胞检测到。此外，乙酰胆碱可通过作用于-氨基-正丁酸/甘氨酸能终末上的烟碱受体而诱导-氨基-正丁酸/甘氨酸的释放。
Spinal-cord slices from neonatal rats were used to record lamina-X neurons using the patch-clamp technique under whole cell recording configuration. Lamina-X surrounds the central canal of the spinal cord and contains sympathetic preganglionic neurons of the central autonomic nucleus. Miniature inhibitory postsynaptic currents were recorded in the presence of tetrodotoxin and kynurenic acid to block action potential-dependent transmitter release and glutamatergic transmissions, respectively. We recorded mixed &ggr;-amino-n-butyric acid/glycine miniature synaptic currents suggesting that &ggr;-amino-n-butyric acid and glycine can be coreleased from the same single synaptic vesicles, and that this corelease can be detected by the postsynaptic cell. In addition, acetylcholine can induce the release of &ggr;-amino-n-butyric acid/glycine by acting presynaptically at nicotinic receptors located on the &ggr;-amino-n-butyric acid ergic/glycinergic terminals.
DOI：
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发表时间：
2004
期刊：
The Journal of comparative neurology
影响因子：
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作者：
C. Patte-Mensah;T. Penning;A. Mensah-Nyagan
通讯作者：
C. Patte-Mensah;T. Penning;A. Mensah-Nyagan