Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae
Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae
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DOI：
10.1016/j.jsb.2016.01.004
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发表时间：
2016-03-01
影响因子：
3
通讯作者：
Gronemeyer, Thomas
中科院分区：
文献类型：
--
作者：
Brausemann, Anton;Gerhardt, Stefan;Gronemeyer, Thomas
Septins是一个保守的GTP结合蛋白家族，在酿酒酵母细胞中，Septins在母芽颈部组装成高度有序的丝状体。酿酒酵母已经被发现了。然而，结构信息只能从建模的结晶亚基的人septins到EM cryomicroscopy数据的酵母异八聚体septin棒。八聚体棒是酵母中Septin细丝的构建块。我们在这里提出的第一个晶体结构的Cdc 11，八聚体杆的末端亚基，并讨论其结构与人类同源物。尺寸排阻色谱分析表明，Cdc 11通过其C-末端卷曲螺旋尾形成同源二聚体。(C)2016 Elsevier Inc. All rights reserved.
Septins are a conserved family of GTP-binding proteins that assemble into a highly ordered array of filaments at the mother bud neck in Saccharomyces cerevisiae cells.Many molecular functions and mechanisms of the septins in S. cerevisiae were already uncovered. However, structural information is only available from modeling the crystallized subunits of the human septins into the EM cryomicroscopy data of the yeast hetero-octameric septin rod. Octameric rods are the building block of septin filaments in yeast. We present here the first crystal structure of Cdc11, the terminal subunit of the octameric rod and discuss its structure in relation to its human homologues. Size exclusion chromatography analysis revealed that Cdc11 forms homodimers through its C-terminal coiled coil tail. (C) 2016 Elsevier Inc. All rights reserved.