Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations
Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations
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DOI：
10.1038/ismej.2017.186
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发表时间：
2017-10
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通讯作者：
W. Qin;Shady A. Amin;Rachel A Lundeen;K. Heal;Willm Martens-Habbena;S. Turkarslan;H. Urakawa;Kyle C Costa;E. Hendrickson;Tony Wang;David AC Beck;Sonia M. Tiquia-Arashiro;F. Taub;Andrew D Holmes;Neeraja Vajrala;P. Berube;Todd M Lowe;James W. Moffett;A. Devol;N. Baliga;D. Arp;L. Sayavedra-Soto;M. Hackett;E. Armbrust;A. Ingalls;David A. Stahl
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作者：
W. Qin;Shady A. Amin;Rachel A Lundeen;K. Heal;Willm Martens-Habbena;S. Turkarslan;H. Urakawa;Kyle C Costa;E. Hendrickson;Tony Wang;David AC Beck;Sonia M. Tiquia-Arashiro;F. Taub;Andrew D Holmes;Neeraja Vajrala;P. Berube;Todd M Lowe;James W. Moffett;A. Devol;N. Baliga;D. Arp;L. Sayavedra-Soto;M. Hackett;E. Armbrust;A. Ingalls;David A. Stahl
氨氧化古生菌(AOA)在海洋系统中的高代表性与它们对氨的高度亲和力、高效的固碳和以铜(Cu)为中心的呼吸系统是一致的。然而，人们对它们对营养胁迫的反应知之甚少。因此，我们使用全局转录和蛋白质组学分析来表征AOA模型Nitrosopumilus maritimusSCM1对氨饥饿、铜限制和铜过量的反应。大多数预测的蛋白质编码基因在指数生长的细胞中转录，在蛋白质组中检测到的~74%的基因中，~6%的基因被N-末端乙酰化修饰。对氨饥饿和铜胁迫的总体反应是能量产生和生物合成的基因下调。细胞对氨饥饿的反应迅速耗尽了氨单加氧酶A和B亚基的转录本，但保留了相对较高的C亚基的转录本。因此，与氨氧化细菌类似，在饥饿过程中选择性地保留amo转录本对于随后的恢复似乎很重要，也表明AMO亚单位转录率可以用来评估海洋种群的生理状态。出人意料的是，钴胺的生物合成在氨饥饿和铜胁迫下都被上调，这表明这种辅因子在胁迫期间保持功能完整性的重要性。
High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA,Nitrosopumilus maritimusSCM1, to ammonia starvation, Cu limitation and Cu excess. Most predicted protein-coding genes were transcribed in exponentially growing cells, and of ~74% detected in the proteome, ~6% were modified by N-terminal acetylation. The general response to ammonia starvation and Cu stress was downregulation of genes for energy generation and biosynthesis. Cells rapidly depleted transcripts for the A and B subunits of ammonia monooxygenase (AMO) in response to ammonia starvation, yet retained relatively high levels of transcripts for the C subunit. Thus, similar to ammonia-oxidizing bacteria, selective retention ofamoCtranscripts during starvation appears important for subsequent recovery, and also suggests that AMO subunit transcript ratios could be used to assess the physiological status of marine populations. Unexpectedly, cobalamin biosynthesis was upregulated in response to both ammonia starvation and Cu stress, indicating the importance of this cofactor in retaining functional integrity during times of stress.