Cytoplasmic expression of SOX9 as a poor prognostic factor for oral squamous cell carcinoma.
Cytoplasmic expression of SOX9 as a poor prognostic factor for oral squamous cell carcinoma.
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DOI：
10.3892/or.2018.6665
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发表时间：
2018-11
期刊：
影响因子：
4.2
通讯作者：
Tanuma JI
中科院分区：
文献类型：
--
作者：
Sumita Y;Yamazaki M;Maruyama S;Abé T;Cheng J;Takagi R;Tanuma JI
转录因子SRY-box 9（SOX 9）是软骨细胞分化和性别决定的关键调节因子，也参与各种类型人类癌症的进展。然而，其与口腔鳞状细胞癌（OSCC）的假定关联仍然难以捉摸。本研究的目的是调查SOX 9在各种口腔上皮病变，包括口腔鳞状细胞癌的表达谱。我们进行了SOX 9表达的免疫组织化学分析，同时表现出不同级别的上皮病变的OSCC的手术标本，并分析了SOX 9的表达与几个临床病理因素之间的相关性。此外，我们进行了免疫荧光染色，蛋白质印迹分析和实时逆转录聚合酶链反应，以评估SOX 9在OSCC HSC-3（转移细胞系）和HSC-4（非转移细胞系）细胞系中的表达。在手术标本中，SOX 9表达在上皮异型增生和原位癌区域的增殖细胞核中检测到，但在正常上皮区域中未检测到。在大多数SCC细胞中观察到核SOX 9表达。值得注意的是，胞质SOX 9表达仅在一些SCC细胞中得到证实;然而，胞质SOX 9表达与不良临床结局显著正相关。HSC-3细胞系中SOX 9的蛋白和mRNA表达均显著高于HSC-4细胞系。然而，值得注意的是，只有HSC-3细胞表现出SOX 9表达的细胞质定位。我们的研究结果表明，SOX 9可能参与了肿瘤的发生和发展的OSCC。此外，其胞质表达代表了肿瘤侵袭性和OSCC预后的潜在预测生物标志物。
Transcription factor SRY-box 9 (SOX9) is a key regulator of chondrocyte differentiation and sex determination, and it is also involved in the progression of various types of human cancer. However, its putative association with oral squamous cell carcinoma (OSCC) remains elusive. The aim of the present study was to investigate the expression profiles of SOX9 in various oral epithelial lesions, including OSCC. We performed immunohistochemical analysis of SOX9 expression in surgical specimens of OSCC, which simultaneously exhibited different grades of epithelial lesions, and analyzed the correlation between SOX9 expression and several clinicopathological factors. Moreover, we performed immunofluorescent staining, western blot analysis and real-time reverse transcription-polymerase chain reaction to assess SOX9 expression in OSCC HSC-3 (a metastatic cell line) and HSC-4 (a non-metastatic cell line) cell lines. In surgical specimens, SOX9 expression was detected in the nuclei of proliferating cells in areas with epithelial dysplasia and carcinoma in situ, but not in areas with normal epithelia. Nuclear SOX9 expression was observed in most SCC cells. Notably, cytoplasmic SOX9 expression was confirmed only in some SCC cells; however, cytoplasmic SOX9 expression was significantly and positively correlated with poor clinical outcomes. Both protein and mRNA expression of SOX9 were significantly higher in the HSC-3 cell line than that in the HSC-4 line. Notably, however, only HSC-3 cells exhibited cytoplasmic localization of SOX9 expression. Our findings indicate that SOX9 may be involved in the tumorigenesis and progression of OSCC. Furthermore, its cytoplasmic expression represents a potential predictive biomarker for tumor aggressiveness and OSCC prognosis.
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通讯作者：
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