Ruthenium-based metal organic framework (Ru-MOF)-derived novel Faraday-cage electrochemiluminescence biosensor for ultrasensitive detection of miRNA-141
Ruthenium-based metal organic framework (Ru-MOF)-derived novel Faraday-cage electrochemiluminescence biosensor for ultrasensitive detection of miRNA-141
复制标题
钌基金属有机骨架（Ru-MOF）衍生的新型法拉第笼电化学发光生物传感器，用于超灵敏检测 miRNA-141
DOI：
10.1016/j.snb.2018.04.088
复制
发表时间：
2018-09-01
影响因子：
8.4
通讯作者：
Guo, Zhiyong
中科院分区：
文献类型：
--
作者：
Shao, Huili;Lu, Jing;Guo, Zhiyong
构建了一种灵敏检测miRNA-141的新型法拉第笼电化学发光（ECL）生物传感器。捕获单元是将捕获DNA （cDNA）固定在功能化的磁性纳米球nanoFe(3)O(4)@SiO2@Au上制备的，信号单元是由信号DNA （sDNA）标记的钌基金属有机骨架Ru-MOF。识别支架为：将捕获单元固定在磁电极表面；捕获目标miRNA-141；信号单元进一步杂交；最后形成了法拉第笼型生物传感器结构。在这种情况下，信号单元成为电极表面的一部分，所提出的电极的外亥姆霍兹平面（OHP）得到扩展，信号单元中的所有电化学发光团都可以参与电极反应，从而大大提高了检测灵敏度。利用提出的法拉第笼级联，发现ECL强度随着miRNA-141浓度的对数而增加。线性范围为1fm ~ 10pm，检测限为0.3 fM。验证了该传感器的选择性、稳定性、重现性、精密度和应用前景。该法拉第笼ECL生物传感器在临床miRNA检测中具有潜在的应用前景。(c) 2018 Elsevier B.V.版权所有
A novel Faraday-cage electrochemiluminescence (ECL) biosensor for the sensitive detection of miRNA-141 was constructed. The capture unit was prepared by immobilizing the capture DNA (cDNA) on the functionalized magnetic nanospheres nanoFe(3)O(4)@SiO2@Au, while the signal unit was ruthenium-based metal organic framework Ru-MOF labeled by signal DNA (sDNA). The recognition scaffold was as follows: the capture unit was immobilized onto the magnetic electrode surface; the target miRNA-141 was caught; the signal unit was further hybridized; the Faraday-cage biosensor structure was formed finally. In this case, the signal unit became part of the electrode surface, the outer Helmholtz plane (OHP) of the proposed electrode was extended, all electrochemiluminophores in the signal unit could take part in the electrode reaction, and thus then the detection sensitivity was greatly improved. Taking advantage of the proposed Faraday-cage cascade, the ECL intensity was found to increase with the logarithm of miRNA-141 concentration. The linear range was wide from 1 fM to 10 pM with a limit of detection 0.3 fM. The selectivity, stability, reproducibility, precision and application of this biosensor were validated. This proposed Faraday-cage ECL biosensor has a potential prospect for clinical miRNA detection. (c) 2018 Elsevier B.V. All rights reserved.