Genome-wide identification and functional analysis of the cyclic nucleotide-gated channel gene family in Chinese cabbage
Genome-wide identification and functional analysis of the cyclic nucleotide-gated channel gene family in Chinese cabbage
复制标题
大白菜环核苷酸门控通道基因家族的全基因组鉴定及功能分析
DOI：
10.1007/s13205-019-1647-2
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发表时间：
2019-03-01
期刊：
影响因子：
2.8
通讯作者：
Liu, Zhiyong
中科院分区：
文献类型：
--
作者：
Li, Qingqing;Yang, Siqiang;Liu, Zhiyong
环核苷酸门控通道（CNGC）是一类广泛存在于动物和植物中的非选择性阳离子通道。植物CNGC参与从发育到胁迫耐受的多种生物学功能。大部分的CNGC基因已经在植物基因组中得到了鉴定，但尚未对大白菜进行过全面的研究。在这项研究中，30 BrCNGC基因被确定，分为五组，并用于进化分析。我们根据与A.拟南芥CNGC。所有BrCNGC基因均随机分布在染色体上，A08染色体不携带任何CNGC基因。大白菜和A.同一类群的拟南芥具有相似的保守基序和基因结构。特别是同源性越近，相似性越高。定量表达分析表明，大部分CNGC基因在4种胁迫下均有表达，表明它们在大白菜的胁迫响应中起着关键作用。12BrCNGC在根、茎、叶、花和角果中的表达模式表明，BrCNGC 8和BrCNGC 16仅在花中特异表达，而在其它部位不表达。本研究为进一步研究大白菜CNGC基因家族的功能奠定了理论基础。
Cyclic nucleotide-gated channels (CNGCs) are a class of nonselective cationic channels that are widely found in animals and plants. Plant CNGCs participate in numerous biological functions that vary from development to stress tolerance. MostCNGCgenes have been identified in plant genomes, but no such comprehensive study has yet been conducted on Chinese cabbage. In this study, thirtyBrCNGCgenes were identified, divided into five groups, and used for evolutionary analysis. We assigned names of all individualCNGCmembers on the basis of phylogenetic relationship withA. thaliana CNGCs. AllBrCNGCgenes were randomly distributed on chromosomes, and the A08 chromosome did not carry anyCNGCgene. TheCNGCgenes of Chinese cabbage andA. thalianafrom the same group displayed similar conserved motifs and gene structures. Especially the closer the homology, the higher the similarity. Quantitative expression analysis showed that most of theCNGCgenes were expressed under four stresses, indicating that they play a key role in the stress response of Chinese cabbage. Expression patterns of 12BrCNGCin the roots, stems, leaves, flowers, and siliques showed thatBrCNGC8andBrCNGC16were specifically expressed only in flowers but not in other parts. This study lays a theoretical foundation for future research on the function of the CNGC gene family in Chinese cabbage.