RGD-modified acellular bovine pericardium as a bioprosthetic scaffold for tissue engineering
RGD-modified acellular bovine pericardium as a bioprosthetic scaffold for tissue engineering
复制标题
RGD 修饰的脱细胞牛心包作为组织工程的生物假体支架
DOI：
10.1007/s10856-009-3791-4
复制
发表时间：
2009-11-01
影响因子：
3.7
通讯作者：
Yi, Dinghua
中科院分区：
文献类型：
--
作者：
Dong, Xiaochao;Wei, Xufeng;Yi, Dinghua
脱细胞生物组织，包括牛心包（BP），已被提议作为组织工程的天然生物材料。然而，天然细胞提取后，其孔径小、孔隙率低、缺乏细胞外基质（extracellular matrix，ECM）等缺陷直接限制了种子细胞的粘附、迁移和增殖，这是ABP在组织工程心脏瓣膜（tissue engineered heart valve，TEHV）中应用的关键问题。在本研究中，我们用乙酸处理脱细胞BP，这增加了支架的孔径和孔隙率，并将RGD多肽缀合到ABP支架上。体外培养10 d后，人骨髓间充质干细胞（hMSCs）在RGD修饰的脱细胞支架上贴壁最好，增殖最快，细胞已深入支架内生长。相反，低密度的细胞附着在未修饰的支架上，很少浸润到脱细胞组织中。这些发现支持了修饰的脱细胞BP作为组织工程心脏瓣膜支架的潜在用途。
Acellular biological tissues, including bovine pericardia (BP), have been proposed as natural biomaterials for tissue engineering. However, small pore size, low porosity and lack of extra cellular matrix (ECM) after native cell extraction directly restrict the seed cell adhesion, migration and proliferation and which is a vital problem for ABP’s application in the tissue engineered heart valve (TEHV). In the present study, we treated acellular BP with acetic acid, which increased the scaffold pore size and porosity and conjugated RGD polypeptides to ABP scaffolds. After 10 days of culture in vitro, the human mesenchymal stem cells (hMSCs) attached the best and proliferated the fastest on RGD-modified acellular scaffolds, and the cell has grown deep into the scaffold. In contrast, a low density of cells attached to the unmodified scaffolds, with few infiltrating into the acellular tissues. These findings support the potential use of modified acellular BP as a scaffold for tissue engineered heart valves.