Susceptibility of high-risk human papillomavirus type 16 to clinical disinfectants
Susceptibility of high-risk human papillomavirus type 16 to clinical disinfectants
复制标题
DOI：
10.1093/jac/dku006
复制
发表时间：
2014-06-01
影响因子：
5.2
通讯作者：
Robison, Richard
中科院分区：
文献类型：
--
作者：
Meyers, Jordan;Ryndock, Eric;Robison, Richard
人们对人乳头瘤病毒 (HPV) 对消毒的敏感性知之甚少。据估计，HPV 是人类最常见的性传播疾病之一。 HPV 也是宫颈癌和其他肛门生殖器癌症的病原体，并导致很大一部分口咽癌。虽然性传播有据可查，但垂直传播和非性传播也可能很重要。使用重组 HPV16 颗粒（准病毒颗粒）和在三维器官型人类上皮培养物中生长的真实 HPV16，我们测试了高危 HPV 对临床消毒剂的敏感性。将感染性病毒颗粒与11种临床常用消毒剂一起孵育，加入适当的中和剂使消毒剂失活，溶液过滤离心。与未处理的病毒相比，测量了经过消毒剂处理的病毒的感染滴度的变化。HPV16是一种高度耐药的病毒；比之前测试的其他无包膜病毒更严重。 HPV16 准病毒体表现出与天然病毒体类似的抵抗力，除了对异丙醇、三酚和较低浓度的过乙酸银（PAA-银）消毒剂敏感之外。真病毒和准病毒对戊二醛和邻苯二甲醛具有抗性，对次氯酸盐和较高浓度的 PAA 银基消毒剂敏感。我们首次提供了 HPV16 天然病毒体的消毒剂敏感性数据，该数据表明常用的临床消毒剂，包括在医疗和牙科保健机构中用作消毒剂的消毒剂，具有 对 HPV16 感染性没有影响。需要改变有关消毒剂使用的政策。 HPV16 对消毒的异常高抵抗力支持了其他数据，表明 HPV16 可能通过污染物或非性传播。
Little to nothing is known about human papillomavirus (HPV) susceptibility to disinfection. HPV is estimated to be among the most common sexually transmitted diseases in humans. HPV is also the causative agent of cervical cancers and other anogenital cancers and is responsible for a significant portion of oropharyngeal cancers. While sexual transmission is well documented, vertical and non-sexual transmission may also be important.Using recombinant HPV16 particles (quasivirions) and authentic HPV16 grown in three-dimensional organotypic human epithelial culture, we tested the susceptibility of high-risk HPV to clinical disinfectants. Infectious viral particles were incubated with 11 common clinical disinfectants, appropriate neutralizers were added to inactivate the disinfectant and solutions were filter centrifuged. Changes in the infectivity titres of the disinfectant-treated virus were measured compared with untreated virus.HPV16 is a highly resistant virus; more so than other non-enveloped viruses previously tested. The HPV16 quasivirions showed similar resistance to native virions, except for being susceptible to isopropanol, the triple phenolic and the lower concentration peracetic acid-silver (PAA-silver)-based disinfectant. Authentic virus and quasivirus were resistant to glutaraldehyde and ortho-phthalaldehyde and susceptible to hypochlorite and the higher concentration PAA-silver-based disinfectant.We present the first disinfectant susceptibility data on HPV16 native virions, which show that commonly used clinical disinfectants, including those used as sterilants in medical and dental healthcare facilities, have no effect on HPV16 infectivity. Policy changes concerning disinfectant use are needed. The unusually high resistance of HPV16 to disinfection supports other data suggesting the possibility of fomite or non-sexual transmission of HPV16.