Systematic Identification of the Genes Affecting Glycogen Storage in the Yeast Saccharomyces cerevisiae
Systematic Identification of the Genes Affecting Glycogen Storage in the Yeast Saccharomyces cerevisiae
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DOI：
10.1074/mcp.m100024-mcp200
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发表时间：
2002-03
影响因子：
7
通讯作者：
W. Wilson;Zhong Wang;P. Roach
中科院分区：
文献类型：
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作者：
W. Wilson;Zhong Wang;P. Roach
在营养限制开始时，酿酒酵母会合成糖原，作为碳源和能量储备。我们利用酵母基因组缺失计划产生的菌株缺失集合，对影响糖原积累的基因进行了系统的调查。分析的菌株集合包含约4600个二倍体纯合子零缺失，占所有可行的单倍体干扰物∼的88%。我们鉴定了324株糖原储存低的菌株和242株糖原储存增加的菌株，占所分析基因的12.4%。通过鉴定许多已知的影响糖原积累的基因，这一筛选得到了验证。许多突变体可以被归入连贯的家族。例如，195或60%的低蓄积者携带与呼吸功能有关的突变，这是一类众所周知的糖原储存缺陷的突变。第二大组由涉及囊泡运输和液泡功能的∼60基因组成，其中包括编码参与液泡ATPase结构或组装的17种蛋白质中的13种的基因。这些数据与我们最近的发现一致，即自噬过程对糖原储存有显著影响(Wang，Z.，Wilson，W.A.，Fujino，M.A.和Roach，P.J.(2001))Snf1p，AMP激活的蛋白激酶的酵母同系物，以及细胞周期蛋白依赖的激酶Pho85p对自噬和糖原积累的拮抗控制。摩尔。牢房。比奥尔。21,5742-5752)。自噬将糖原运送到液泡，我们认为与ATPase突变体(vma10或vma22)相关的空泡功能受损导致糖原降解减少和随后的过度积累。
At the onset of nutrient limitation, the yeast Saccharomyces cerevisiae synthesizes glycogen to serve as a carbon and energy reserve. We undertook a systematic survey for the genes that affect glycogen accumulation by taking advantage of the strain deletion set generated by the Saccharomyces Genome Deletion Project. The strain collection analyzed contained some 4600 diploid homozygous null deletants, representing ∼88% of all viable haploid disruptants. We identified 324 strains with low and 242 with elevated glycogen stores, accounting for 12.4% of the genes analyzed. The screen was validated by the identification of many of the genes known already to influence glycogen accumulation. Many of the mutants could be placed into coherent families. For example, 195 or 60% of the hypoaccumulators carry mutations linked to respiratory function, a class of mutants well known to be defective in glycogen storage. The second largest group consists of ∼60 genes involved in vesicular trafficking and vacuolar function, including genes encoding 13 of 17 proteins involved in the structure or assembly of the vacuolar ATPase. These data are consistent with our recent findings that the process of autophagy has a significant impact on glycogen storage (Wang, Z., Wilson, W. A., Fujino, M. A., and Roach, P. J. (2001) Antagonistic controls of autophagy and glycogen accumulation by Snf1p, the yeast homolog of AMP-activated protein kinase, and the cyclin-dependent kinase Pho85p. Mol. Cell. Biol. 21, 5742–5752). Autophagy delivers glycogen to the vacuole, and we propose that the impaired vacuolar function associated with ATPase mutants (vma10 or vma22) results in reduced degradation and subsequent hyperaccumulation of glycogen.