Involvement of gangliosides and glycoprotein fibronectin receptors in cellular adhesion to fibronectin.
Involvement of gangliosides and glycoprotein fibronectin receptors in cellular adhesion to fibronectin.
复制标题
神经节苷脂和糖蛋白纤连蛋白受体参与细胞粘附纤连蛋白。
DOI：
10.1016/0014-4827(88)90027-4
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发表时间：
1988
影响因子：
3.7
通讯作者：
Stallcup,WB
中科院分区：
文献类型：
--
作者：
Stallcup,WB
我们使用大鼠神经细胞系B65，研究神经节苷脂和糖蛋白受体在纤维连接蛋白粘附中的相对贡献。针对两种神经外胚层相关神经节苷脂D1.1和GD3的单克隆抗体抑制B65附着到纤连蛋白的速率，表明这些神经节苷脂参与粘附过程。与纤连蛋白的粘附不受第三种单克隆抗体的影响，该抗体针对B65细胞的单独的、未鉴定的细胞表面组分。此外，缺乏D1.1的B65细胞以比表达D1.1的B65细胞更慢的速率粘附于纤连蛋白。糖蛋白受体参与粘附通过抗人纤连蛋白受体的抗体抑制B65与纤连蛋白附着的能力来证明。此外，粘附被含有Arg → Gly → Asp纤连蛋白序列的六肽阻断，该序列是与受体结合所必需的。在不存在二价阳离子的情况下胰蛋白酶处理B65细胞导致纤连蛋白受体的蛋白水解，伴随着细胞附着于纤连蛋白的能力的丧失。D1.1和GD3的表达不受胰蛋白酶消化的影响，表明神经节苷脂本身不能介导附着。糖蛋白受体必须主要负责与纤维连接蛋白的粘附，神经节苷脂作为增强剂或调节剂发挥次要作用。
We have used a rat neural cell line, B65, to investigate the relative contributions of gangliosides and glycoprotein receptors in adhesion to fibronectin. Monoclonal antibodies against two neuroectoderm-associated gangliosides, D1.1 and GD3, inhibit the rate of B65 attachment to fibronectin, suggesting that these gangliosides are involved in the adhesion process. Adhesion to fibronectin is not affected by a third monoclonal antibody against a separate, unidentified cell-surface component of B65 cells. Furthermore, B65 cells lacking D1.1 adhere to fibronectin at a slower rate than B65 cells that express D1.1. The involvement of glycoprotein receptors in adhesion is demonstrated by the ability of antibodies against human fibronectin receptor to inhibit B65 attachment to fibronectin. In addition, adhesion is blocked by a hexapeptide containing the ArgGlyAsp fibronectin sequence which is necessary for binding to the receptor. Trypsin treatment of B65 cells in the absence of divalent cations results in proteolysis of the fibronectin receptor with an accompanying loss of ability of the cells to attach to fibronectin. D1.1 and GD3expression is not affected by this trypsinization, indicating that the gangliosides alone are incapable of mediating attachment. The glycoprotein receptors must be primarily responsible for adhesion to fibronectin with the gangliosides playing a secondary role as enhancers or modulators.